Recombinant Saccharomyces cerevisiae Inositol-3-phosphate synthase (INO1), partial

Code CSB-YP318913SVG
MSDS
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Source Yeast
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Code CSB-EP318913SVG
MSDS
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Source E.coli
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Code CSB-EP318913SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318913SVG
MSDS
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Source Baculovirus
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Code CSB-MP318913SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
INO1
Uniprot No.
Alternative Names
INO1; YJL153C; J0610Inositol-3-phosphate synthase; MIP synthase; EC 5.5.1.4; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Data suggest that a specific pool of phosphatidic acid, associated with lipid droplet formation in perinuclear ER, is responsible for initial rapid exit of Opi1p from nucleus to ER and is required for Ino1 expression in presence of choline; interaction between Opi1p and Scs2p in ER membrane facilitates transit of phosphatidic acid from ER to mitochondria. PMID: 28924045
  2. Transcriptional memory of INO1 requires a remodeled form of the Set1/COMPASS methyltransferase lacking Spp1, which dimethylates histone H3 lysine 4 (H3K4me2). PMID: 27336723
  3. analysis of how myo-inositol-3-phosphate synthase is regulated by phosphorylation PMID: 23902760
  4. While the pattern of regulation of these two genes was similar, results showed that intermediate levels of inositol repressed INO1 and induced SNA3. PMID: 23174187
  5. Data show that the introns have a direct role in enhancing transcription of INO1 and ASC1 because there was a marked increase in nascent transcripts from these genes in the presence of an intron. PMID: 22586116
  6. Interaction of a DNA zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. PMID: 20932479
  7. Induction of INO1 resulted in acetylation of both histones H3 and H4 at the INO1 promoter and sequences farther downstream in the coding region, suggesting a gene-wide acetylation in response to transcriptional activation. PMID: 20018175
  8. Full transcriptional activation of INO1 and another GRS-containing gene requires GRS I (gene recruitment sequences) mediated targeting of the promoter to the nuclear periphery. PMID: 20098417
  9. unfolded protein response pathway induction not correlated with elevated INO1 expression PMID: 15371354
  10. the intranuclear localization of INO1 can profoundly influence its mechanism of activation PMID: 15455074
  11. cells with down-regulated Sah1 activity major alterations in the expression of the INO1 gene as well as in the localization of Opi1, a negative regulatory factor of phospholipid synthesis PMID: 18591246

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Subcellular Location
Cytoplasm.
Protein Families
Myo-inositol 1-phosphate synthase family
Database Links

KEGG: sce:YJL153C

STRING: 4932.YJL153C

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