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Recombinant Saccharomyces cerevisiae Iron transport multicopper oxidase FET3 (FET3), partial

Recombinant Saccharomyces cerevisiae Iron transport multicopper oxidase FET3 (FET3), partial

Code	CSB-YP340040SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP340040SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP340040SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP340040SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP340040SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

FET3

Uniprot No.

P38993

Alternative Names

FET3; YMR058W; YM9796.11Iron transport multicopper oxidase FET3; EC 1.-.-.-

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Iron transport multicopper ferroxidase required for Fe(2+) ion high affinity uptake. Required to oxidize Fe(2+) and release it from the transporter. Essential component of copper-dependent iron transport.

Gene References into Functions

Presence of crowding agents does not affect Fet3p structural content but increases thermal resistance. The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics. PMID: 19932772
The D94 residue appears to activate a proton of the type 2 Cu(+)-bound water for participation in the transition state. PMID: 15835897
Substitution of Asp320 with asparagine essentially abolished the Fet3-dependent iron transport activity. All other mutants (D278Q, D279N, D312N and D315I) behaved essentially as the wild-type protein. PMID: 16032772
results are consistent with a (minimal) heterodimer model of the Fet3p.Ftr1p complex that supports the trafficking of iron from Fet3p to Ftr1p for iron permeation across the yeast plasma membrane PMID: 16522632
D283, E185, and D409 in Fet3p provide a Fe(II) binding site that actually favors ferric iron. PMID: 17042492
Structure-function analysis of the cuprous oxidase activity in Fet3p from Saccharomyces cerevisiae PMID: 17220296
We find that unfolding reactions of apo and different holo forms of Fet3p are irreversible reactions that depend on the scan rate and there is a metal-induced tradeoff between overall protein stability and metal coordination PMID: 19033465