Recombinant Saccharomyces cerevisiae JmjC domain-containing histone demethylation protein 1(JHD1)

Code CSB-YP336665SVG
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Source Yeast
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Code CSB-EP336665SVG
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Source E.coli
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Code CSB-EP336665SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336665SVG
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Source Baculovirus
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Code CSB-MP336665SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names JHD1
Uniprot No. P40034
Alternative Names
JHD1; YER051W; JmjC domain-containing histone demethylation protein 1; EC 1.14.11.27; Jumonji/ARID domain-containing protein 1; ScJHDM1; [Histone-H3]-lysine-36 demethylase 1
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region 1-492
Target Protein Sequence MQDPNICQHC QLKDNPGALI WVKCDSCPQW VHVKCVPLKR IHYSNLTSSE VLSYPNSAKQ IKSYRCPNHK EGEYLTAYAL ITQKGKRQRN KENPEDSHIN KRYNFRKKKL LDYIALNEGE SKRDKMNHPH KESFMKSFEK WKNGSNIINA ADFAEKFDNI DVPYKIIDPL NSGVYVPNVG TDNGCLTVNY ITEMIGEDYH VDVMDVQSQM NENWNLGSWN EYFTNTEPDR RDRIRNVISL EVSNIEGLEL ERPTAVRQND LVDKIWSFNG HLEKVNGEKA EENDPKPKVT KYILMSVKDA YTDFHLDFAG TSVYYNVISG QKKFLLFPPT QSNIDKYIEW SLKEDQNSVF LGDILEDGIA MELDAGDLFM IPAGYIHAVY TPVDSLVFGG NFLTIRDLET HLKIVEIEKL TKVPRRFTFP KFDQVMGKLC EYLALDKNKI TSDVSDGDLL SRTTNCAIQS LHAYVIKPEV KYKPLNFTSK KHLAKALADL IS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Does not demethylate H3 'Lys-4' nor 'Lys-79'.
Gene References into Functions
  1. These studies establish Jhd1 as a histone demethylase in budding yeast and suggest that Jhd1 functions to maintain the fidelity of histone methylation patterns along transcription units. PMID: 17470555
  2. Jhd1 and Rph1 demethylate histone H3 Lys36 in transcribed regions to promote elongation PMID: 17525156
  3. Possible role for jhd1 enzyme, through inhibition by succinate in paraganglioma oncogenesis. PMID: 17884808
Subcellular Location Nucleus.
Protein Families JHDM1 histone demethylase family
Database Links

KEGG: sce:YER051W

STRING: 4932.YER051W

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