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Recombinant Saccharomyces cerevisiae Kinetochore protein NDC80 (TID3), partial

Recombinant Saccharomyces cerevisiae Kinetochore protein NDC80 (TID3), partial

Code	CSB-YP331173SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP331173SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP331173SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP331173SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP331173SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

NDC80

Uniprot No.

P40460

Alternative Names

NDC80; HEC1; TID3; YIL144W; Kinetochore protein NDC80; 80 kDa spindle component protein; Nuclear division cycle protein 80; Two-hybrid interaction with DMC1 protein 3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.

Gene References into Functions

Thus, expression of a 5' extended mRNA isoform causes transcriptional interference at the downstream promoter of NDC80. The authors demonstrate that this is an effective mechanism to promote dynamic changes in gene expression during cell differentiation. PMID: 28906248
The authors discovered that in budding yeast, kinetochore inactivation during meiosis occurs by reducing the abundance of a limiting subunit, Ndc80. PMID: 28906249
Ndc80 helical hairpin, microtubule-binding and the tetramerization domains are essential for Ndc80 function. PMID: 23833183
We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential.Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint. PMID: 19822728
data on the four-protein Ndc80 complex suggests that the Spc24p/Spc25p end of the rod faces the centromere and the Ndc80p/Nuf2p end faces a spindle microtubule PMID: 15809444
Results suggest that the conformational kink in Ndc80 is essential for correct kinetochore geometry and could be part of a tension-sensing mechanism at the kinetochore. PMID: 18793650
Findings demonstrate how an ensemble of Ndc80 complexes may provide the combination of plasticity and strength that allows kinetochores to maintain load-bearing tip attachments during both microtubule assembly and disassembly. PMID: 19269365