Recombinant Saccharomyces cerevisiae Mitochondrial genome maintenance protein MGM101 (MGM101)

Code CSB-YP329199SVG
MSDS
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Source Yeast
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Code CSB-EP329199SVG
MSDS
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Source E.coli
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Code CSB-EP329199SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329199SVG
MSDS
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Source Baculovirus
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Code CSB-MP329199SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MGM101
Uniprot No.
Alternative Names
MGM101; MGM9; YJR144W; J2181Mitochondrial genome maintenance protein MGM101
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
31-269
Target Protein Sequence
TATAGAVRKS FNSTETKPVF ATKSEAGNGS HMKEYSSGIN SKLGGTPLET RSTADDSLNN SYKQVKGDID WYTSWYGLGM KPFEAKVQKD LIEPLDPKDI EIKPDGLIYL PEIKYRRILN KAFGAGGWGL VPRSQTIVTS KLVTREYGLI CHGQLISVAR GEQDYFNEAG IPTATEGCKS NALMRCCKDL GVGSELWDPV FIKKFKVDHC TEKFVEHVTT KRKKKIWLRK DRQVEYPYK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Performs an essential function in the repair of oxidatively damaged mtDNA that is required for the maintenance of the mitochondrial genome. Binds to DNA.
Gene References into Functions
  1. The carboxy terminal-tail of Mgm101, likely displayed on the ring surface, is required for single stranded DNA binding. PMID: 23536705
  2. Data indicate that Mgm101p is found in the mitochondrial nucleoid along with enzymes and proteins involved in mtDNA replication. PMID: 23418572
  3. A potential role of Cys-216 and Cys-217 in regulating Mgm101 function and the repair of damaged mtDNA under stress conditions. PMID: 22948312
  4. Mgm101 is a Rad52-related protein required for mitochondrial DNA recombination PMID: 22027892
  5. a previously identified recessive temperature-sensitive mgm101-1ts mutation was used as a detection system to examine the functionality of Mgm101p PMID: 17311591

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Subcellular Location
Mitochondrion matrix, mitochondrion nucleoid.
Protein Families
MGM101 family
Database Links

KEGG: sce:YJR144W

STRING: 4932.YJR144W

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