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Recombinant Saccharomyces cerevisiae Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1 (YME1), partial

Recombinant Saccharomyces cerevisiae Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1 (YME1), partial

Code	CSB-YP335768SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP335768SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP335768SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP335768SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP335768SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

YME1

Uniprot No.

P32795

Alternative Names

YME1; OSD1; YTA11; YPR024W; YP9367.04; Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1; EC 3.4.24.-; Protein OSD1; Tat-binding homolog 11; Yeast mitochondrial escape protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Catalytic subunit of the mitochondrial inner membrane i-AAA protease supercomplex required for mitochondrial inner membrane protein turnover. The protease is probably ATP-dependent. Important to maintain the integrity of the mitochondrial compartment. Required both for the degradation of unassembled subunit 2 of cytochrome c oxidase (COX2) and for efficient assembly of mitochondrial respiratory chain. Binds unfolded substrates in an ATPase-independent manner; binding of folded COX2, a physiological substrate, requires an active ATPase but when COX2 is destabilized an active ATPase is no longer necessary.

Gene References into Functions

this study presents an atomic model of a substrate-bound inner mitochondrial membrane AAA+ quality control protease in yeast, YME1. PMID: 29097521
Yme1 role in Tim10 degradation PMID: 26182355
identify an important role for the mitochondrial protease Yme1 in the ability of cells that lack tafazzin function to maintain mitochondrial structural integrity and mitochondrial quality control and to undergo mitophagy PMID: 25688091
No individual cysteine residue is required for the function of Tim9 or Tim10 in yeast and defective assembly of the small TIMs induces their proteolytic clearance from mitochondria via the mitochondrial ATP-dependent protease, Yme1. PMID: 23036860
Inactivation of either Yme1 or Yta10/12, allows fermentative growth of hsc82 or hsp82 strains at 37 degrees C. PMID: 21439406
Yme1 has a new function in protein translocation, indicating that the intermembrane space harbors diverse pathways for protein translocation. PMID: 16966379
There are two distinct substrate binding sites in the i-AAA protease subunit Yme1. PMID: 17261594
The phosphatidylethanolamine level of yeast mitochondria is affected by the mitochondrial components OXA1 and YME1. PMID: 17976194

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Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note=Although this protein does not have any predicted transmembrane helices it behaves like an integral membrane protein.

Protein Families

AAA ATPase family; Peptidase M41 family

Database Links

KEGG: sce:YPR024W

STRING: 4932.YPR024W

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