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Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1 (BFA1), partial

Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1 (BFA1), partial

Code	CSB-YP344036SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP344036SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP344036SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP344036SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP344036SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

BFA1

Uniprot No.

P47113

Alternative Names

BFA1; YJR053W; J1667Mitotic check point protein BFA1; Cell cycle arrest protein BFA1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.

Gene References into Functions

Tem1 activity is tightly controlled in mitosis by Bub2 and Bfa1 and is also regulated by the spindle orientation checkpoint that monitors the correct alignment of the mitotic spindle with the mother-daughter axis. Here is described the purification of Tem1, Bfa1, and Bub2 and a detailed radioactive filter-binding assay to study the nucleotide binding properties of Tem1 and the role of its regulators Bfa1 and Bub2. PMID: 27826857
Bmh1 is a spindle position checkpoint (SPOC) component that breaks Bfa1 centrosome asymmetry upon SPOC activation. PMID: 24850890
Cdc5 induces the asymmetric distribution of Bfa1 to the bud-directed spindle pole body independently of Bfa1 GAP activity at anaphase. PMID: 22253605
various checkpoint signals are transmitted to the C-terminal domain of Bfa1 (Bfa1-D8(391-574)) and Bfa1p localization to spindle pole body is necessary but not sufficient to regulate mitotic exit in response to various checkpoint signals PMID: 15147270
Bfa1 disappearance from the mother bound spindle pole body at the onset of anaphase, along with Cdc5 function, helps prompt Tem1 activation in telophase. PMID: 16449187
Results suggest that the GTPase-activating activity of Bfa1p-Bub2p is responsible for the mitotic arrest caused by spindle damage and Bfa1p overproduction. PMID: 16682821
there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles PMID: 18667533
A link is confirmed between the subcellular localization pattern of Bfa1 at the spindle pole bodies with cell cycle progression. PMID: 19948498