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Recombinant Saccharomyces cerevisiae Oligomycin resistance ATP-dependent permease YOR1 (YOR1), partial

Recombinant Saccharomyces cerevisiae Oligomycin resistance ATP-dependent permease YOR1 (YOR1), partial

Code	CSB-YP345644SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP345644SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP345644SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP345644SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP345644SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

YOR1

Uniprot No.

P53049

Alternative Names

YOR1; YGR281W; Oligomycin resistance ATP-dependent permease YOR1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Functions as a pleiotropic drug pump at the plasma membrane to clear toxic substances from the cytosol. Organic anion transporter involved in the detoxification of a wide range of toxic environmental organic anions that contain carboxyl groups. Required for tolerance to reveromycin A, tautomycin and leptomycin B. Required for oligomycin resistance. Required for rhodamine B resistance. Mediates the ATP-dependent efflux of rhodamine B. Involved in cadmium detoxification. Displays an energy-dependent efflux of cadmium and glutathione, suggesting that YOR1 transports both compounds as a bis-glutathionato-cadmium Cd-(GS)(2) complex. Confers resistance to rhodamine 6G and to doxorubicin.

Gene References into Functions

Data reveal that it is possible to modulate activity of ABC transporters Yor1p by physically replacing dysfunctional domains. PMID: 20843810
Strains expressing Yor1p display an energy-dependent efflux of cadmium estimated for the yeasts overexpressing Yor1p to be about 0.02 nmol 109Cd/mg protein/min. PMID: 16814918
Endoplasmic reticulum quality control checkpoint engages misfolded Yor1p even after it has been stabilized by inhibition of the degradative pathway. PMID: 17615300
Yor1p also confers resistance to rhodamine 6G and to doxorubicin. PMID: 17950691
Mapping of interdomain interfaces required for the functional architecture of Yor1p, a eukaryotic ATP-binding cassette (ABC) transporter PMID: 18644782

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily

Database Links

KEGG: sce:YGR281W

STRING: 4932.YGR281W

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