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Recombinant Saccharomyces cerevisiae Oligopeptide transporter 1 (OPT1), partial

Recombinant Saccharomyces cerevisiae Oligopeptide transporter 1 (OPT1), partial

Code	CSB-YP334785SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP334785SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP334785SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP334785SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP334785SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

OPT1

Uniprot No.

P40897

Alternative Names

OPT1; GSH11; HGT1; YJL212C; HRD799; J0236; Oligopeptide transporter 1; High affinity glutathione transporter 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

High affinity transporter for glutathione. Also transports tetra- and pentapeptides like the opioids leucine enkephalin (Tyr-Gly-Gly-Phe-Leu) and methionine enkephalin (Tyr-Gly-Gly_Phe-Met) across the cell membrane.

Gene References into Functions

Data suggest that protonated residues E/D135 or N710 in Hgt1p prevent substrate/glutathione from binding at high pH; mutation of these residues allows substrate/glutathione to bind in a proton-dependent manner through involvement of other residues. PMID: 28389436
ScOPT1 and AtOPT4 are proton-coupled OPTs with broad but distinct substrate specificities and affinities. PMID: 16149917
The cysteine-free Hgt1p was nonfunctional and pointed to a critical role being played by the native cysteine residues of Hgt1p. PMID: 19496824
Asn-124 in transmembrane domain 1(TMD1), Gln-222 in TMD4, Gln-526 in TMD9, and Glu-544, Arg-554, and Lys-562 in the intracellular loop are essential for the transport activity of Hgt1. PMID: 19589778

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Oligopeptide OPT transporter family

Database Links

KEGG: sce:YJL212C

STRING: 4932.YJL212C

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