Recombinant Saccharomyces cerevisiae Peptide methionine sulfoxide reductase (MXR1)

Code CSB-YP331115SVG
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP331115SVG
MSDS
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Source E.coli
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Code CSB-EP331115SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331115SVG
MSDS
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Source Baculovirus
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Code CSB-MP331115SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MXR1
Uniprot No.
Alternative Names
MXR1; YER042W; Peptide methionine sulfoxide reductase; EC 1.8.4.11; Peptide-methionine; S)-S-oxide reductase; Peptide Met(O) reductase; Protein-methionine-S-oxide reductase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-184
Target Protein Sequence
MSSLISKTIK YDPAKDKLIT LACGCFWGTE HMYRKYLNDR IVDCKVGYAN GEESKKDSPS SVSYKRVCGG DTDFAEVLQV SYNPKVITLR ELTDFFFRIH DPTTSNSQGP DKGTQYRSGL FAHSDADLKE LAKIKEEWQP KWGNKIATVI EPIKNFYDAE EYHQLYLDKN PQGYACPTHY LREM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. Also able to reduce dimethyl sulfoxide (DMSO) as well, with DMS as the product.
Gene References into Functions
  1. the plasticity of the Trx binding site on Mxr1 provides structural insights into the recognition of diverse substrates by a universal catalytic motif of Trx. PMID: 21345799
  2. under normal growth conditions thioredoxin 1 is essential for msrA transcription and activity PMID: 16631529
  3. interacts with glutathione peroxidase Gpx3; Gpx3 functions as a redox-dependent exquisite regulator of the protein repair activity of Mxr1 PMID: 16808898
  4. Functional analysis of free methionine-R-sulfoxide reductase from Saccharomyces cerevisiae. PMID: 19049972
  5. MSRA and MSRB helps to preserve the function of cellular FeS clusters from oxidative inactivation. PMID: 19202110

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Protein Families
MsrA Met sulfoxide reductase family
Database Links

KEGG: sce:YER042W

STRING: 4932.YER042W

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