Recombinant Saccharomyces cerevisiae Peroxisomal targeting signal 2 receptor (PEX7)

Code CSB-YP017808SVG
MSDS
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Source Yeast
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Code CSB-EP017808SVG
MSDS
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Source E.coli
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Code CSB-EP017808SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017808SVG
MSDS
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Source Baculovirus
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Code CSB-MP017808SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEX7
Uniprot No.
Alternative Names
PEX7; PAS7; PEB1; YDR142C; YD2943.01C; YD9302.18C; Peroxisomal targeting signal 2 receptor; PTS2 receptor; Peroxin-7; Peroxisome import protein PAS7
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-375
Target Protein Sequence
MLRYHMQGFS GYGVQYSPFF DNRLAVAAGS NFGLVGNGKL FILEIDRSGR IVEVNSFLTQ DCLFDLAWNE SHENQVLVAQ GDGTLRLFDT TFKEFPIAIF KEHEREVFSC NWNLVNRQNF LSSSWDGSIK IWSPLRKQSL MTLTPRPLEI TKMVDPLNAI ILKKKSFTGI SKNRNCVYQA QFSPHDQNLV LSCSGNSYAS LFDIRLPSGK NQNNFLVHSG LEALTCDFNK YRPYVVATGG VDNAIRIWDI RMLNKNESAT IKRTVPGQLH NSSCINEIPN AHGLAIRKVT WSPHHSNILM SASYDMTCRI WRDLSNDGAK ETYKTNSTDA TKGSIFNFTQ HSEFVFGADW SLWGKPGYVA STAWDGNLFV WNGLG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to the N-terminal PTS2-type peroxisomal targeting signal and plays an essential role in peroxisomal protein import essential for import of 3-oxoacyl-CoA thiolase (a PTS2-containing protein) into peroxisomes. May direct thiolase to peroxisomes by shuttling between the cytosol and peroxisomal membranes.
Gene References into Functions
  1. appending GFP to Pex7p's COOH terminus shifts Pex7p's intracellular distribution from predominantly cytosolic to predominantly peroxisomal in Saccharomyces cerevisiae PMID: 15545321
  2. the C terminus of Pex14p contains distinct Pex5p and one Pex7p binding sites and the N terminus could be involved in a Pex5p-Pex14p association inside the peroxisomal membrane PMID: 16107331
  3. Data strongly suggest that the formation of the ternary cytosolic PTS2 pre-import complex occurs hierarchically. PMID: 19183303
Subcellular Location
Cytoplasm. Peroxisome.
Protein Families
WD repeat peroxin-7 family
Database Links

KEGG: sce:YDR142C

STRING: 4932.YDR142C

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