Product Details

Purity

>85% (SDS-PAGE)

Target Names

PAH1

Uniprot No.

P32567

Alternative Names

PAH1; PAP1; SMP2; YMR165C; YM8520.14CPhosphatidic acid phosphohydrolase 1; PAP1; EC 3.1.3.4; Protein SMP2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. Required for de novo lipid synthesis and formation of lipid droplets. Controles transcription of phospholipid biosynthetic genes and nuclear structure by regulating the amount of membrane present at the nuclear envelope. Involved in plasmid maintenance, in respiration and in cell proliferation.

Gene References into Functions

Site-specific mutational analyses of the conserved residues within WRDPLVDID indicated that Trp-637 plays a crucial role in Pah1 function. This work also demonstrated that the catalytic activity of Pah1 is required but is not sufficient for its in vivo functions PMID: 29066621
These results suggest that Pah1p plays an important role in vacuolar activity by negatively regulating the expression of V-ATPase genes. As such, we provide evidence to show the role of Pah1p in vacuolar acidification and fragmentation. PMID: 28756231
The expression of Pah1 with combined mutations of S705D and 7A, which mimic its phosphorylation by CKII and lack of phosphorylation by Pho85-Pho80, caused an increase in triacylglycerol content and lipid droplet number in cells expressing the Nem1-Spo7 phosphatase complex. PMID: 27044741
shortened chronological life span of the pah1Delta mutant along with its growth defect on non-fermentable carbon sources and hypersensitivity to hydrogen peroxide was suppressed by the loss of Dgk1 diacylglycerol kinase PMID: 26338708
proteolysis of Pah1 by the 20S proteasome is controlled by its phosphorylation state PMID: 25809482
Report functional conservation of PAP1 between yeast and human. PMID: 25475986
essential role of PAH1 in triacylglycerol synthesis throughout growth PMID: 24196957
Phosphorylation/dephosphorylation is a major mechanism by which Pah1p PAP activity is regulated. (Review) PMID: 22910056
Pah1p and PA phosphatase activity are critical for vacuole homeostasis and fusion. PMID: 22121197
the expression of PAH1-encoded phosphatidate phosphatase was induced by zinc deficiency. PMID: 22128164
Phosphatidate phosphatase activity plays key role in protection against fatty acid-induced toxicity in yeast PMID: 21708942
Data suggest that diacylglycerol generated by Pah1p is important for lipid droplet biogenesis. PMID: 21422231
Dephosphorylation of Pah1p by the Nem1p-Spo7p complex enables the amphipathic helix to anchor Pah1p onto the nuclear/ER membrane allowing the production of diacylglycerol for lipid biosynthesis. PMID: 20876142
Smp2 associates with the promoters of phospholipid biosynthetic enzymes in a Nem1-Spo7-dependent manner. Smp2 is a critical factor in coordinating phospholipid biosynthesis at the nuclear/ER membrane with nuclear growth during the cell cycle. PMID: 15889145
reports for the first time the identification of the S. cerevisiae PAH1 (previously known as SMP2) gene encoding a Mg2+-dependent PA phosphatase enzyme PMID: 16467296
PAP1 activity of the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology. PMID: 17971454

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Subcellular Location

Cytoplasm. Nucleus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Protein Families

Lipin family

Database Links

KEGG: sce:YMR165C

STRING: 4932.YMR165C

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Code	CSB-MP333712SVG
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Recombinant Saccharomyces cerevisiae Phosphatidic acid phosphohydrolase 1 (PAH1), partial

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