Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRP43

Uniprot No.

P53131

Alternative Names

PRP43; YGL120C; Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43; EC 3.6.4.13; Helicase JA1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.

Gene References into Functions

These data indicate that the U2 snRNP-intron interaction is Prp43's major target. PMID: 27115347
Using Prp43 F357A mutants or pyrimidine nucleotides, we show that the lack of stacking of the nucleotide base to the F-motif decouples the NTPase and helicase activities of Prp43. In contrast the R159A mutant (R-motif) showed reduced ATPase and helicase activities. PMID: 28180308
Study shows the crystal structure of Prp43p bound to single-stranded poly-uracil RNA and reveals differences with viral ATPases in the RecA1 domain but with striking similarities with viral ATPases in the RecA2 domain of the catalytic core, including both a prominent beta-hairpin that bookends the 5' end of the RNA stack and a dynamic motif Va that is implicated in mediating translocation. PMID: 28416566
Results show that Prp43 expression is regulated by Cmg1 which stimulates its RNA helicase activity. PMID: 26821976
Prp43 differentially interacts with g-patch domains in Spp382, Sqs1, and Pxr1 G. PMID: 25808954
This study investigated the activation of Prp43 by Ntr1 in the presence and absence of RNA substrate. PMID: 24165877
The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing. PMID: 20705241
Prp43p promotes the discard of suboptimal and optimal 5' exon and lariat intermediates indiscriminately. PMID: 20463285
Data report that cleavage of pre-rRNA at the 3'-end of 18 S rRNA, requires functional interactions between Nob1 and Prp43, and the cofactor Pfa1. PMID: 19801658
stimulation of ATPase/helicase activities of Prp43p by Pfa1p is required for efficient 20S pre-rRNA-to-18S rRNA conversion PMID: 19927118
The ATPase activity of Prp43p is required for early steps of pre-rRNA processing and normal accumulation of mature rRNAs. PMID: 16227579
Prp43p promotes recycling of snoRNAs and biogenesis factors during pre-rRNA processing. The dual function for Prp43p in the cell raises the possibility that ribosome biogenesis and pre-mRNA splicing may be coordinately regulated. PMID: 16382143
Its essential function is in its newly characterized role in ribosome biogenesis of both ribosomal subunits, positioning Prp43p to regulate both pre-mRNA splicing and ribosome biogenesis. PMID: 16382144
We report that Prp43 hydrolyzes all common NTPs and dNTPs and unwinds short 5'/3' tailed RNA/DNA duplexes in an ATP-dependent fashion. PMID: 16700561
Prp43 acts on preribosomal regions surrounding each binding site, with distinct functions at different locations. PMID: 19941819

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Subcellular Location

Nucleus.

Protein Families

DEAD box helicase family, DEAH subfamily, DDX15/PRP43 sub-subfamily

Database Links

KEGG: sce:YGL120C

STRING: 4932.YGL120C

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Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (PRP43), partial

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