Product Details

Purity

>85% (SDS-PAGE)

Target Names

GAL3

Uniprot No.

P13045

Alternative Names

GAL3; YDR009W; YD8119.14; Protein GAL3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.

Gene References into Functions

Computational study allowed to correctly predict that a non-synonymous SNP would change the binding affinity of Gal3p with the Gal80p transcriptional repressor. PMID: 29335276
The results suggest that the dynamics of residue F237 situated in the hydrophobic pocket located in the hinge region drives the transition between different conformations. PMID: 27913116
GAL3 is the major driver of GAL induction variation, and that GAL3 allelic variation alone can explain as much as 90% of the variation in GAL induction in a cross. PMID: 28542190
Discuss the effect of Gal3 on the quaternary structure of Gal80 in light of the evidence pointing to multimeric Gal80 as the form required to inhibit Gal4. PMID: 23858060
Overproduction of Gal3p or Gal4p mutants with altered Gal80p-binding characteristics restores GAL gene expression in mutants deficient in ATP or NADP(H) synthesis. PMID: 20979343
the binding of Gal3 to the Gal80 monomer competes with Gal80 self-association, reducing the amount of the Gal80 dimer available for inhibition of Gal4 PMID: 15695361
a conserved tyrosine in Gal3 has a role in signal transduction PMID: 16160853
Gal1p and Gal3p respond to their substrates in a similar, perhaps identical, fashion PMID: 16603548
Gal4p and Gal3p bind to Gal80p at distinct but overlapping sites PMID: 17121853
A reverse two-hybrid selection for GAL3 mutations that impair the Gal3-Gal80 interaction yielded 12 single-amino-acid substitutions at residues that are predicted to be surface exposed on Gal3. PMID: 18245852
Gal3p moves from the cytoplasm to the nucleus and interact with Gal80p in response to galactose induction. PMID: 18952899
A model of the structure, based on the crystal structure of the highly similar Gal1p, permits the mapping of point mutations which affect transcription activation. A model for Gal3p-Gal80p action is proposed. PMID: 16115868
Both MgATP and galactose are required to promote the formation of a 1:1 complex between Gal3p and Gal80p. PMID: 11964151

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Protein Families

GHMP kinase family, GalK subfamily

Database Links

KEGG: sce:YDR009W

STRING: 4932.YDR009W

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Recombinant Saccharomyces cerevisiae Protein GAL3 (GAL3), partial

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