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Recombinant Saccharomyces cerevisiae Protein OS-9 homolog (YOS9), partial

Recombinant Saccharomyces cerevisiae Protein OS-9 homolog (YOS9), partial

Code	CSB-YP860329SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP860329SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP860329SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP860329SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP860329SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

YOS9

Uniprot No.

Q99220

Alternative Names

YOS9; YDR057W; D4222; Protein OS-9 homolog

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation. The recognition of targets is N-glycan specific. Functions in recruiting misfolded protein substrates in conjunction with HRD3.

Gene References into Functions

Yos9p and Hrd1p mediate ER retention of misfolded proteins in the early stage of ERAD, which constitutes a process separable from the later degradation step. PMID: 22298424
Structural and biochemical basis of Yos9 protein dimerization and possible contribution to self-association of 3-hydroxy-3-methylglutaryl-coenzyme A reductase degradation ubiquitin-ligase complex. PMID: 22262864
Data show that Yos9 slows the degradation of the unglycosylated ERAD substrate CPY *0000. PMID: 21871892
Yos9p has a previously unrecognized role in the quality control of nonglycosylated proteins PMID: 21737688
Data suggest that Yos9p plays a critical role in the bipartite recognition of terminally misfolded glycoproteins. PMID: 16168370
Data show that Yos9p binds directly to substrates to discriminate misfolded from folded proteins. PMID: 16168371
Data show that Yos9 protein in Saccharomyces cerevisiae is essential for efficient degradation of mutant glycoproteins. PMID: 16168372
Yos9p ensures that only terminally misfolded polypeptides are degraded. PMID: 16845381