Recombinant Saccharomyces cerevisiae Protein phosphatase 2C homolog 6 (PTC6)

Code CSB-YP333093SVG
MSDS
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Source Yeast
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Code CSB-EP333093SVG
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Source E.coli
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Code CSB-EP333093SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333093SVG
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Source Baculovirus
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Code CSB-MP333093SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PTC6
Uniprot No.
Alternative Names
PTC6; AUP1; PPP2; YCR079W; YCR79C; YCR79W; [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2; mitochondrial; PDP 2; EC 3.1.3.16; EC 3.1.3.43; Autophagy-related protein phosphatase 1; Phosphatase two C protein 6; Protein phosphatase 2C homolog 6; PP2C-6; Protein phosphatase of PDH protein 2; Pyruvate dehydrogenase complex phosphatase 2; PDC phosphatase 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
35-442
Target Protein Sequence
SRINRL ENQDNYSIKM MRSWPNAYGS ALNCSVFDGH GEKGAQLSQL LADKLCSSLD FPEPSWDKQD LKKLVQEYAR RFPEGNYWKH KLSTFEKFYN KFIKNCNSKQ ELLLMKEGDS AILGQNGGRM IFDKMGNIID KIALLTELDR LRLFYGFARF DLDQCCGLGT AAGSTASSIF LYPYDDPNAP IDEGKDDDSW IISHSGLLKL IVTQVGDSKI ILCDQDGIAH ALTTTHHINS SRERHRLSID PSRLDPDAFG ETRFLNNFAN TRSFGDVAGK PYGISSEPDI FSFLVGNTLH LPRSERSKLP FNGDECFLAL VTDGITNKLA DQEVVDLITS TVNSWGLKKA TPQFVAEETI KFIQAIATKH SDNATCVVVR LSNWGNWPNV DRTGPQRETK LMNAQSNETK LN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein phosphatase that shows typical type 2C protein phosphatase (PP2C) activity. Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex. Required for efficient mitophagy in stationary phase cells.
Gene References into Functions
  1. Data suggest that Ptc6p is the primary pyruvate dehydrogenase phosphatase in S. cerevisiae; Ptc6p exhibits substrate specificity for additional phosphatases. PMID: 28539364
  2. S. cerevisiae PTC6 participates in the negative regulation of Slt2 phosphorylation and vacuole morphogenesis under cell wall stress conditions PMID: 25449759
  3. encodes the sixth type 2C protein phosphatase in Saccharomyces cerevisiae and confers rapamycin-resistant function. PMID: 17002782
  4. Pkp2p (Ygl059wp) and Ppp2p (Ycr079wp), are engaged in the regulation of this complex by affecting the phosphorylation state of Pda1p. PMID: 18180296
  5. the function of Aup1 in mitophagy could be explained through its regulation of Rtg3-dependent transcription. PMID: 19840933

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Subcellular Location
Mitochondrion intermembrane space. Mitochondrion matrix.
Database Links

KEGG: sce:YCR079W

STRING: 4932.YCR079W

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