Product Details

Purity

>85% (SDS-PAGE)

Target Names

INO80

Uniprot No.

P53115

Alternative Names

INO80; YGL150C; G1880Chromatin-remodeling ATPase INO80; EC 3.6.4.-; Inositol-requiring protein 80

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating with 'Ser-129' phosphorylated H2A histones as a response to DNA damage.

Gene References into Functions

INO80 translocates along DNA at the H2A-H2B interface of nucleosomes and persistently displaces DNA from the surface of H2A-H2B. The data suggest that INO80 has a mechanism for dimer exchange that is distinct from other chromatin remodellers including its paralogue SWR1. PMID: 28604691
The INO80 complex is a central component of metabolic homeostasis that influences histone acetylation and may contribute to disease when disrupted. PMID: 29462149
Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in S. cerevisiae. PMID: 28735899
Arp5Delta, Ies6Delta, and INO80Delta mutants demonstrate decreased expression of genes involved in glycolysis and increased expression of genes in the oxidative phosphorylation pathway. PMID: 26755556
Results identify INO80 as a bona fide component of the proteolytic pathway for RNAPII degradation and suggest that INO80 nucleosome remodeling activity promotes the dissociation of ubiquitinated Rpb1 from chromatin to protect the integrity of the genome. PMID: 26656161
Arp5-Ies6 interacts with chromatin via assembly with the INO80 complex, as IES2 and INO80 deletion results in loss of Arp5-Ies6 chromatin association PMID: 26306040
INO80 can activate Rad53 kinase activity in an Ies4-phosphorylation-dependent manner in the absence of known activators such as Rad9. PMID: 25959398
Both remodelers, Ino80p and Snf2p are required to recruit RNA polymerase II and targeted histone acetylation, indicating that remodelers are recruited to the CUP1 promoter before RNA polymerase II and histone acetylases. PMID: 24471920
INO80 chromatin remodeler is shown to act downstream from Mec1 to increase chromatin mobility, highlighting an additional damage-related role of this nucleosome remodeling complex PMID: 24029917
Interactions between the nucleosome histone core and Arp8 in the INO80 chromatin remodeling complex PMID: 23213201
INO80 maintains genome stability. PMID: 23207916
these data provide a mechanism for cross talk within transcriptional co-activators Ino80p and Snf2p during INO1 activation. PMID: 22281492
Isw2 and Ino80 are both enriched at stalled replication forks and physically and specifically interact with a single-stranded DNA binding protein, replication protein A PMID: 21930788
INO80 moved arrays of two or three nucleosomes with 50 or 79 bp of linker DNA closer together, with a final length of approximately 30 bp of linker DNA or a repeat length of approximately 177 bp. PMID: 21135121
In the absence of INO80, H2A.Z nucleosomes are mislocalized, and H2A.Z levels at promoters show reduced responsiveness to transcriptional changes, suggesting that INO80 controls H2A.Z dynamics. PMID: 21241891
These results demonstrate a critical role of INO80 in creating DNA accessibility for the NER pathway and provide direct evidence that repair of UV lesions and perhaps most bulky adduct lesions requires chromatin reconfiguration. PMID: 20855601
INO80 complex is required for the proper establishment of sister chromatid cohesion. PMID: 17471029
Ies3p subunit of the Saccharomyces cerevisiae INO80 chromatin remodeling complex interacts with a conserved tetratricopeptide repeat domain of the telomerase protein Est1p. PMID: 17562861
The Ies4 subunit of the INO80 complex is phosphorylated by the Mec1/Tel1 kinases during exposure to DNA-damaging agents PMID: 17693258
enriched at stalled replication forks, where it promotes the resumption of replication upon recovery from fork arrest PMID: 18406137
SWI/SNF and INO80 are present at the INO1 promoter and are restricted to the promoter, indicating that they directly participate in localized INO1 chromatin remodeling. PMID: 18593569
Chromatin remodeling by INO80 in cooperation with Spt6 determines the shape of the expression profile under acute stress conditions, possibly by an elongation-dependent mechanism. PMID: 19620280

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Subcellular Location

Nucleus.

Protein Families

SNF2/RAD54 helicase family

Database Links

KEGG: sce:YGL150C

STRING: 4932.YGL150C

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Recombinant Saccharomyces cerevisiae Putative DNA helicase INO80 (INO80), partial

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