Recombinant Saccharomyces cerevisiae RNA annealing protein YRA1 (YRA1)

Code CSB-YP618573SVG
MSDS
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Source Yeast
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Code CSB-EP618573SVG
MSDS
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Source E.coli
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Code CSB-EP618573SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618573SVG
MSDS
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Source Baculovirus
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Code CSB-MP618573SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YRA1
Uniprot No.
Alternative Names
YRA1; YDR381W; D9481.2; D9509.1; RNA annealing protein YRA1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-226
Target Protein Sequence
SANLDKSLD EIIGSNKAGS NRARVGGTRG NGPRRVGKQV GSQRRSLPNR RGPIRKNTRA PPNAVARVAK LLDTTREVKV NVEGLPRDIK QDAVREFFAS QVGGVQRVLL SYNERGQSTG MANITFKNGE LARRAVERFN GSPIDGGRSR LRLNLIVDPN QRPVKSLADR IKAMPQKGGN APRPVKRGPN RKAAMAKSQN KPKREKPAKK SLEDLDKEMA DYFEKK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding RNA annealing protein. May have a role in pre-mRNA metabolism. Component the TREX complex, which operates in coupling transcription elongation to mRNA export.
Gene References into Functions
  1. Yra1 binds RNA-DNA hybrids in vitro and, when artificially overexpressed, can be recruited to chromatin in an RNA-DNA hybrid-dependent manner, stabilizing R loops and converting them into replication obstacles in vivo PMID: 29954833
  2. YRA1 overexpression causes a cell senescence-like phenotype and telomere shortening PMID: 27035147
  3. These data show that Yra1 inhibits unwinding in a concentration-dependent manner by preventing the association of Dbp2 with single-stranded RNA. PMID: 26876600
  4. the phospho-CTD is likely involved directly in the cotranscriptional recruitment of Yra1. PMID: 21856751
  5. Data indicate that Edc3p-mediated YRA1 pre-mRNA decay occurs independently of translation and also requires Mex67p. PMID: 20463951
  6. We propose that appropriate levels of Yra1p are maintained by inefficient cotranscriptional splicing. PMID: 16618971
  7. YRA1p can bind to nascent mRNAs, heat shock RNA was exported efficiently in temperature-sensitive YRA1 mutant strains PMID: 17259545
  8. YRA1 exon 1 sequences in cis and Yra1p in trans inhibit YRA1 pre-mRNA splicing and commit the pre-mRNA to nuclear export. PMID: 17317628
  9. Dia2 binding to replication origins is significantly reduced when association with Yra1 is compromised PMID: 17452447
  10. Nap1 interacts directly with Yra1. The recruitment of Nap1 to the coding region of actively transcribed genes is Yra1 dependent. PMID: 18227150
  11. Yra1 binds directly to the Zn finger/Clp1 region of Pcf11, the pol II CTD-binding subunit of CF1A. PMID: 19110458

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YDR381W

STRING: 4932.YDR381W

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