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Recombinant Saccharomyces cerevisiae Regulator of Ty1 transposition protein 107 (RTT107), partial

Recombinant Saccharomyces cerevisiae Regulator of Ty1 transposition protein 107 (RTT107), partial

Code	CSB-YP327899SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP327899SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP327899SVG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP327899SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP327899SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

RTT107

Uniprot No.

P38850

Alternative Names

RTT107; ESC4; YHR154W; Regulator of Ty1 transposition protein 107; Establishes silent chromatin protein 4

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.

Gene References into Functions

A key function of the Rtt107 BRCT domains for targeting of both itself and its interaction partners to DNA lesions was revealed. PMID: 26641499
The DNA repair scaffold proteins Slx4 and Rtt107 utilize a minimal multi-BRCT-domain module for phosphatase-independent downregulation of DNA damage response signals in yeast. PMID: 25896509
The C-terminal tandem BRCT repeats of Rtt107 interaction with gammaH2A is functionally important during DNA repair. PMID: 22262834
a multifaceted functional relationship between Rtt107 and Dot1 in the DNA damage response and maintenance of genome integrity. PMID: 20810656
identify Esc4p as an important new S-phase-specific target of monitors and participates in meiotic recombination Mec1p PMID: 14988729
Slx4-dependent phosphorylation of Rtt107 by Mec1 is critical for replication restart after alkylation damage. PMID: 16267268
Esc4 binds the Rad55/Rad56 heterodimer and forms subnuclear foci in response to DNA damage that stalls replication forks. PMID: 16569515
Rtt107 is recruited to chromatin following DNA damage that stalls DNA replication forks. This recruitment depends on the acetyltransferase Rtt109 and the cullin Rtt101. Chromatin-bound Rtt107 is localized at or near stalled replication forks. PMID: 17978089