Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase ATG1 (ATG1), partial

Code CSB-YP346821SVG
MSDS
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Source Yeast
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Code CSB-EP346821SVG
MSDS
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Source E.coli
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Code CSB-EP346821SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP346821SVG
MSDS
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Source Baculovirus
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Code CSB-MP346821SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATG1
Uniprot No.
Alternative Names
ATG1; APG1; AUT3; CVT10; YGL180W; G1615Serine/threonine-protein kinase ATG1; EC 2.7.11.1; Autophagy protein 3; Autophagy-related protein 1; Cytoplasm to vacuole targeting protein 10
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Finally, ATG1 is also required for the maintenance of cell viability under starvation and for glycogen storage during stationary phase. Plays a role in genome stability through suppression of abnormal mitosis under starvation, and in regulation of filamentous growth.
Gene References into Functions
  1. Data show that phosphorylation by Atg1 inhibits Atg4 at autophagosome formation sites. PMID: 28821724
  2. The article shows how selective autophagy is tightly regulated in space and time to prevent aberrant Atg1 kinase activation and autophagy induction. PMID: 27768871
  3. Atg1 complex provide insights into assembly and regulatory mechanisms PMID: 25998554
  4. In vitro studies show that Atg1 kinase is activated by selective autophagy targets in yeast requiring the Atg11 scaffold. PMID: 26166702
  5. These results lead to a model for autophagy initiation in which Atg1 and Atg13 are tightly associated with one another and assemble transiently into the pentameric Atg1 complex during starvation. PMID: 25139988
  6. The Atg1 kinase acts early in autophagy by regulating the outgrowth of autophagosomal membranes. PMID: 24440502
  7. An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy. PMID: 21712380
  8. findings suggest that the direct control of the Atg1 complex by Tor complex1 induces autophagy. PMID: 19995911
  9. Data show that Atg1, PKA directly phosphorylates Atg13 in a Tor-dependent manner. PMID: 19805182
  10. Atg1 kinase activity affects the dynamics of protein movement at the phagophore assembly site involved in Atg protein cycling. PMID: 18077553
  11. Atg1 plays a structural role for efficient recruitment of Atg proteins to the pre-autophagosomal structure, which is mediated by interaction with Atg13 and Atg17. PMID: 18552550

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Subcellular Location
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Formes punctate structures in starvation conditions only when ATG13 and ATG17 were both present. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily
Database Links

KEGG: sce:YGL180W

STRING: 4932.YGL180W

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