Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG1 Polyclonal antibody

Uniprot No.

P53104

Target Names

ATG1

Alternative Names

ATG1 antibody; APG1 antibody; AUT3 antibody; CVT10 antibody; YGL180W antibody; G1615Serine/threonine-protein kinase ATG1 antibody; EC 2.7.11.1 antibody; Autophagy protein 3 antibody; Autophagy-related protein 1 antibody; Cytoplasm to vacuole targeting protein 10 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Finally, ATG1 is also required for the maintenance of cell viability under starvation and for glycogen storage during stationary phase. Plays a role in genome stability through suppression of abnormal mitosis under starvation, and in regulation of filamentous growth.

Gene References into Functions

Data show that phosphorylation by Atg1 inhibits Atg4 at autophagosome formation sites. PMID: 28821724
The article shows how selective autophagy is tightly regulated in space and time to prevent aberrant Atg1 kinase activation and autophagy induction. PMID: 27768871
Atg1 complex provide insights into assembly and regulatory mechanisms PMID: 25998554
In vitro studies show that Atg1 kinase is activated by selective autophagy targets in yeast requiring the Atg11 scaffold. PMID: 26166702
These results lead to a model for autophagy initiation in which Atg1 and Atg13 are tightly associated with one another and assemble transiently into the pentameric Atg1 complex during starvation. PMID: 25139988
The Atg1 kinase acts early in autophagy by regulating the outgrowth of autophagosomal membranes. PMID: 24440502
An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy. PMID: 21712380
findings suggest that the direct control of the Atg1 complex by Tor complex1 induces autophagy. PMID: 19995911
Data show that Atg1, PKA directly phosphorylates Atg13 in a Tor-dependent manner. PMID: 19805182
Atg1 kinase activity affects the dynamics of protein movement at the phagophore assembly site involved in Atg protein cycling. PMID: 18077553
Atg1 plays a structural role for efficient recruitment of Atg proteins to the pre-autophagosomal structure, which is mediated by interaction with Atg13 and Atg17. PMID: 18552550

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Subcellular Location

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Formes punctate structures in starvation conditions only when ATG13 and ATG17 were both present. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily

Database Links

KEGG: sce:YGL180W

STRING: 4932.YGL180W

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ATG1 Antibody

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