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Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1 (CBK1), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1 (CBK1), partial

Code	CSB-YP347240SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP347240SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP347240SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP347240SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP347240SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CBK1

Uniprot No.

P53894

Alternative Names

CBK1; YNL161W; N1727Serine/threonine-protein kinase CBK1; EC 2.7.11.1; Cell wall biosynthesis kinase

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Protein kinase that seems to play a role in the regulation of cell morphogenesis and proliferation.

Gene References into Functions

Cbk1 and Bck2 are required for Mpk1 activation during heat shock and cell wall stress and for Mpk1 dephosphorylation during heat shock adaptation. PMID: 22031291
propose that Cdc14 coordinates cell separation with mitotic exit via FEAR-initiated phosphorylation of the Cbk1 HM site and MEN-activated reversal of mitotic CDK phosphorylations that block both Cbk1 and Ace2 function PMID: 21135117
Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control. PMID: 19962308
Mutations in BRR1 or MPT5 lead to a restoration of fertility induced by cbk1p mutation, and a more-or-less pronounced restoration of polarity; they also show genetic interactions with SSD1. PMID: 19546315
CBK1-S745F allele leads to a constitutively active form of Cbk1p that is independent of the upstream RAM network. PMID: 19967545
Phosphoregulation of Cbk1 is critical for RAM network function. Intramolecular autophosphorylation of the enzyme's activation loop is critical for kinase activity but is only partially required for cell separation and polarized growth. PMID: 17145962
Cbk1 exhibits a novel specificity that is likely conserved among related kinases from yeast to metazoans. Cbk1 is functionally restricted to the daughter cell, and cannot diffuse from the daughter to the mother. PMID: 18715118
These data suggest that an important function for Cbk1 and the RAM signaling network is to regulate growth and secretion via Golgi and Sec2/Sec4-dependent processes. PMID: 18843045