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Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase MPS1 (MPS1), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase MPS1 (MPS1), partial

Code	CSB-YP346524SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP346524SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP346524SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP346524SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP346524SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MPS1

Uniprot No.

P54199

Alternative Names

MPS1; RPK1; YDL028C; D2785; Serine/threonine-protein kinase MPS1; EC 2.7.12.2; Monopolar spindle protein 1; Regulatory cell proliferation kinase 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Implicated in spindle pole body (SPD) duplication. Dual specificity kinase that can phosphorylate serine, threonine and tyrosine residues. Phosphorylates the SPC29 and SPC110 spindle pole body components.

Gene References into Functions

An important role for Mps1 phosphorylation in the spindle assembly checkpoint activation, beyond the direct recruitment of Mad1 and Mad2. PMID: 27280788
CCT271850 is a potent, selective and orally bioavailable MPS1 kinase inhibitor. On the basis of in vivo pharmacodynamic vs efficacy relationships, we predict that more than 80% inhibition of MPS1 activity for at least 24 h is required to achieve tumour stasis or regression by CCT271850. PMID: 28334731
These findings highlight MPS1 as a rational therapeutic target for medulloblastoma PMID: 27633003
Disrupting Mps1 before mitotic entry reduces chromosomal passenger complex (CPC) localization to the centromere. Mps1 plays a minimal role in maintaining CPC localization once cells are in mitosis. PMID: 25854549
Mps1 kinase controls Cnn1 localization and activity at kinetochores through the cell cycle. PMID: 25716979
Ipl1 released these improper connections, whereas Mps1 triggered the formation of new force-generating microtubule attachments. PMID: 23371552
found that Sgo1 overexpression rescues defects caused by metaphase inactivation of Mps1 and that Mps1 is required for Sgo1 localization to the kinetochore PMID: 21389114
Mps1 plays an important role in kinetochore attachment during mitotic spindle assembly and function. PMID: 15668173
it was demonstrated that mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the checkpoint, leads to sustained inactivation of the spindle checkpoint PMID: 16825537
Phosphorylation of Dam1 residues S218 and S221 by Mps1 is required for efficient coupling of kinetochores to microtubule plus ends. PMID: 16890524
Mps1 promotes re-orientation of kinetochore-spindle pole connections and eliminates those that do not generate tension between sister kinetochores. PMID: 18060784
Results suggest that a putative Mps1-dependent phosphorylation of Ndc80 is important for spindle assembly checkpoint activation at kinetochores. PMID: 19300438