Recombinant Saccharomyces cerevisiae Suppressor protein STM1 (STM1)

Code CSB-YP334507SVG
MSDS
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Source Yeast
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Code CSB-EP334507SVG
MSDS
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Source E.coli
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Code CSB-EP334507SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334507SVG
MSDS
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Source Baculovirus
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Code CSB-MP334507SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
STM1
Uniprot No.
Alternative Names
STM1; MPT4; STO1; YLR150W; L9634.1; Suppressor protein STM1; 3BP1; GU4 nucleic-binding protein 2; G4p2 protein; POP2 multicopy suppressor protein 4; Ribosomal subunits association factor; AF; TOM1 suppressor protein 1; Triplex-binding protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-273
Target Protein Sequence
SNPFDLLGN DVEDADVVVL PPKEIVKSNT SSKKADVPPP SADPSKARKN RPRPSGNEGA IRDKTAGRRN NRSKDVTDSA TTKKSNTRRA TDRHSRTGKT DTKKKVNQGW GDDKKELSAE KEAQADAAAE IAEDAAEAED AGKPKTAQLS LQDYLNQQAN NQFNKVPEAK KVELDAERIE TAEKEAYVPA TKVKNVKSKQ LKTKEYLEFD ATFVESNTRK NFGDRNNNSR NNFNNRRGGR GARKGNNTAN ATNSANTVQK NRNIDVSNLP SLA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds specifically G4 quadruplex (these are four-stranded right-handed helices, stabilized by guanine base quartets) and purine motif triplex (characterized by a third, antiparallel purine-rich DNA strand located within the major groove of a homopurine stretch of duplex DNA) nucleic acid structures. These structures may be present at telomeres or in rRNAs. Acts with CDC13 to control telomere length homeostasis. Involved in the control of the apoptosis-like cell death.
Gene References into Functions
  1. These findings suggest that Stm1p can act as a ribosome preservation factor under conditions of nutrient deprivation and restoration. PMID: 23206692
  2. Stm1 may recognize the shape of the triplex rather than the base sequence of the triplex. PMID: 18776278
  3. Stm(amino acids 1-113) is the minimal domain to specifically bind with the purine motif triplex. PMID: 18029617
  4. Stm1 loss-of-function alleles and overexpression strains show several genetic interactions with Pat1 and Dhh1 alleles in a manner consistent with Stm1 working upstream of Dhh1 to promote Dhh1 function. PMID: 19015546
  5. data indicates that Stm1p plays a complementary role to eEF3 in translation. PMID: 19666721

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Concentrated in the perinuclear region.
Database Links

KEGG: sce:YLR150W

STRING: 4932.YLR150W

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