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Recombinant Saccharomyces cerevisiae TATA-binding protein-associated factor MOT1 (MOT1), partial

Recombinant Saccharomyces cerevisiae TATA-binding protein-associated factor MOT1 (MOT1), partial

Code	CSB-YP329158SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP329158SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP329158SVG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP329158SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP329158SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MOT1

Uniprot No.

P32333

Alternative Names

MOT1; YPL082C; LPF4C; TATA-binding protein-associated factor MOT1; TBP-associated factor MOT1; EC 3.6.4.-; Modifier of transcription 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box via its C-terminal ATPase activity. Both transcription activation and repression require its ATPase activity.

Gene References into Functions

these results suggest that TBP-DNA affinity as well as other aspects of promoter sequence influence Mot1 function in vivo. PMID: 27255709
The results stress the importance of the cooperation of Mot1p/NC2 with remodelers in maintaining a repressive environment for intragenic transcription. PMID: 24459134
The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites. PMID: 24144978
In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions. PMID: 22298788
findings suggest that the four conserved regions of Mot1 are essential for viability and required for proper regulation of most Mot1-regulated genes; loss of the ATPase domain, however, imparts some unexpected regulation on certain genes PMID: 21444714
Mot1 plays a broad role in establishing the precision and efficiency of RNA synthesis. PMID: 20855454
MOT1 might have a role in vivo as a transcriptional activator in the small ubiquitin-related modifier (SUMO) protein pathway. PMID: 16387868
A novel ATP-independent TBPc displacement activity for Mot1 is reported PMID: 16541100
These results support a model in which Mot1 affects the rate and efficiency of rRNA synthesis by both direct and indirect mechanisms, with resulting effects on transcription activation and the coupling of rRNA synthesis to processing. PMID: 17296733
Mot1p interacts with a range of transcriptional co-regulators as well as proteins involved in chromatin remodeling. PMID: 18596064
Mot1-mediated dissociation of TBP (or TBP-containing complexes) from chromatin can explain the Mot1 activation mechanism at some promoters. PMID: 19098311
These results therefore demonstrate that Mot1 is an in vivo SUMO-targeted ubiquitin ligase target in yeast and suggest a role for SUMO-targeted degradation in protein quality control. PMID: 19139279