Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MOT1 Polyclonal antibody

Uniprot No.

P32333

Target Names

MOT1

Alternative Names

MOT1 antibody; YPL082C antibody; LPF4C antibody; TATA-binding protein-associated factor MOT1 antibody; TBP-associated factor MOT1 antibody; EC 3.6.4.- antibody; Modifier of transcription 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MOT1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box via its C-terminal ATPase activity. Both transcription activation and repression require its ATPase activity.

Gene References into Functions

these results suggest that TBP-DNA affinity as well as other aspects of promoter sequence influence Mot1 function in vivo. PMID: 27255709
The results stress the importance of the cooperation of Mot1p/NC2 with remodelers in maintaining a repressive environment for intragenic transcription. PMID: 24459134
The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites. PMID: 24144978
In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions. PMID: 22298788
findings suggest that the four conserved regions of Mot1 are essential for viability and required for proper regulation of most Mot1-regulated genes; loss of the ATPase domain, however, imparts some unexpected regulation on certain genes PMID: 21444714
Mot1 plays a broad role in establishing the precision and efficiency of RNA synthesis. PMID: 20855454
MOT1 might have a role in vivo as a transcriptional activator in the small ubiquitin-related modifier (SUMO) protein pathway. PMID: 16387868
A novel ATP-independent TBPc displacement activity for Mot1 is reported PMID: 16541100
These results support a model in which Mot1 affects the rate and efficiency of rRNA synthesis by both direct and indirect mechanisms, with resulting effects on transcription activation and the coupling of rRNA synthesis to processing. PMID: 17296733
Mot1p interacts with a range of transcriptional co-regulators as well as proteins involved in chromatin remodeling. PMID: 18596064
Mot1-mediated dissociation of TBP (or TBP-containing complexes) from chromatin can explain the Mot1 activation mechanism at some promoters. PMID: 19098311
These results therefore demonstrate that Mot1 is an in vivo SUMO-targeted ubiquitin ligase target in yeast and suggest a role for SUMO-targeted degradation in protein quality control. PMID: 19139279

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Subcellular Location

Mitochondrion. Nucleus. Note=Localized on chromatin. Specifically localized to the promoters of the genes it regulates.

Protein Families

SNF2/RAD54 helicase family

Database Links

KEGG: sce:YPL082C

STRING: 4932.YPL082C

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MOT1 Antibody

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