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Recombinant Saccharomyces cerevisiae Telomere elongation protein EST1 (EST1), partial

Recombinant Saccharomyces cerevisiae Telomere elongation protein EST1 (EST1), partial

Code	CSB-YP325486SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP325486SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP325486SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP325486SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP325486SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

EST1

Uniprot No.

P17214

Alternative Names

EST1; YLR233C; L8083.15Telomere elongation protein EST1; Ever shorter telomeres protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Directly involved in telomere replication. Associates with telomerase and during its interaction with CDC13, telomerase activity is promoted.

Gene References into Functions

Recruitment of Est1 to telomeres via a Cdc13-Est1 fusion is sufficient to bypass the role of Ku in promoting telomere elongation. PMID: 24879463
Autonomous localization of Est1p to the nucleus via the classical nuclear import pathway contributes to normal telomere length maintenance. PMID: 24906415
Esl1 has no telomere-related functions, but contributes to the regulation of adaptive gene expression responses. PMID: 23893744
It is possible that the intramolecular G-quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo. PMID: 23376615
There is a biochemical interaction between Est1 and the telomere binding protein Cdc13. Association of the Est1 protein with the telomerase catalytic core is not a regulatory event that contributes to telomere homeostasis. PMID: 23390975
the telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro PMID: 21969561
The tricotetrapeptide repeat of Est1 is required for Est2-mediated telomere length maintenance. PMID: 22011238
These results demonstrate that one major functional role that Est1 brings to the telomerase complex is the capping or protection of telomeres. PMID: 21220516
The authors show that the yeast telomerase subunit Est1p, known to be involved in telomerase recruitment to telomeres, can convert single-stranded telomeric G-rich DNA into a G-quadruplex structure in vitro in a Mg(2+)-dependent manner. PMID: 20098422
telomerase assembly during G1 phase is regulated by Est1p stability, but assembly is insufficient to activate telomerase at telomeres PMID: 16862158
dimerization of TLC1 is a functionally conserved feature of the RNA templates utilized by reverse transcriptases of both viral and cellular origins PMID: 17491007
Maintenance of functional levels of Est2p late in the cell cycle requires that Est1p. PMID: 18949040