Product Details

Purity

>85% (SDS-PAGE)

Target Names

SPT6

Uniprot No.

P23615

Alternative Names

SPT6; CRE2; SSN20; YGR116W; G6169; Transcription elongation factor SPT6; Chromatin elongation factor SPT6

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.

Gene References into Functions

Results provide evidence that both FACT and Spt6 are important for transcription globally and may participate during different stages of transcription. PMID: 29695490
Interactions between SPT6 and RNA Polymerase II PMID: 27261007
Results demonstrate that a fine-tuning of the Spt16 component of FACT by UPS is essential for proper chromatin reassembly and efficient transcriptional elongation, thus revealing a novel ubiquitin proteasome system regulation of FACT with physiological relevance in gene expression. PMID: 27044865
Histone chaperones FACT and Spt6 are important for appropriate H2AZ histone localization and for the functioning of RNA polymerase II. PMID: 26990181
Data show that the histone chaperones POB3 protein FACT and chromatin-remodeling histone chaperone Spt6 prevent widespread accumulation of histone H2A.Z. PMID: 25959393
Spt6 is involved in either recruiting the Pol I-Rrn3 complex to the rDNA or stabilizing the preinitiation complex. The findings presented here identify an unexpected, essential role for Spt6 in synthesis of rRNA. PMID: 25918242
Data suggest that phosphorylation of the Pol II C-terminal domain on Tyr1 promotes the association of Spt6 with the 3' ends of transcribed genes, independently of Ser2 phosphorylation. PMID: 25182531
A report on the mechanism involving Spt6 and Ctk1 in the regulation and maintenance of RNA polymerase II Ser2 C-terminal domain phosphorylation during transcription elongation. PMID: 24163256
Spt6 may use the tandem SH2 domain module to sense the phosphorylation level of RNAPII CTD. PMID: 21676864
these findings provide a structural foundation for understanding how Spt6 encodes several distinct functions within a single polypeptide chain. PMID: 21419780
results provide new evidence for a connection between the transcription elongation factor Spt6 and RNA 3'-end formation in vivo PMID: 15531585
Results identify Spt6 as the factor that mediates nucleosome reassembly onto the PHO5 and other promoters during transcriptional repression. PMID: 16455495
These findings link Spn1 functions to the transition from an inactive to an actively transcribing RNAPII complex at a postrecruitment-regulated promoter. PMID: 18086892
The C-terminal region of Set2, in conjunction with the phosphorylated C-terminal domain of RNAPII, influences the KMTase activity to promote methylation of histone H3 at lysine 36 during transcription elongation. PMID: 18541663
The SH2 domain is required for normal expression of a subset of yeast genes, and is consistent with multiple functions of Spt6 in chromatin transcription. PMID: 19371747

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Subcellular Location

Nucleus. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to the active transcribed loci.

Protein Families

SPT6 family

Database Links

KEGG: sce:YGR116W

STRING: 4932.YGR116W

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Code	CSB-YP022960SVG
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Code	CSB-MP022960SVG
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Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT6 (SPT6), partial

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