Recombinant Saccharomyces cerevisiae Transcription initiation factor IIB (SUA7)

Code CSB-YP010001SVG
MSDS
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Source Yeast
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Code CSB-EP010001SVG
MSDS
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Source E.coli
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Code CSB-EP010001SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010001SVG
MSDS
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Source Baculovirus
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Code CSB-MP010001SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SUA7
Uniprot No.
Alternative Names
SUA7; YPR086W; P9513.4; Transcription initiation factor IIB; General transcription factor TFIIB; Transcription factor E
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-345
Target Protein Sequence
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Gene References into Functions
  1. crystal structure of RNA polymerase II-TFIIB complex at 3.8 A resolution revealing carboxyl-terminal region of TFIIB, located above polymerase active center cleft but showing none of B finger; linker between amino- & carboxyl-terminal regions also seen PMID: 19965383
  2. Results describe the architecture of transcription factor IIB and the the RNA polymerase II transcription preinitiation complex (PIC). PMID: 15479635
  3. TBP1(E186D) temperature-sensitivity and slow-growth phenotypes might have been caused by insufficient amounts of Tbp1p for efficient transcription of the rRNA genes by RNA polymerase I PMID: 17680779
  4. TFIIB binds RNA Polymerase II at the terminator, which in turn associates with the promoter scaffold giving TFIIB a role in gene looping. PMID: 17803944
  5. the crystal structure of the complete RNA polymerase II - transcription factor IIB complex at 4.3 A resolution PMID: 19820686

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Subcellular Location
Nucleus.
Protein Families
TFIIB family
Database Links

KEGG: sce:YPR086W

STRING: 4932.YPR086W

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