Recombinant Saccharomyces cerevisiae Ubiquitin-like protein SMT3 (SMT3)

The recombinant Baker's yeast SMT3 protein is encoded by the gene of SMT3 (2-98aa). The gene of SMT3 was cloned in a system (E.coli) that supported the expression of SMT3 and translation of messenger RNA. Modification of SMT3 by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 6xHis-SUMO tag in the production. The purity is 90% determined by SDS-PAGE.

SMT3 is a protein coding gene that encodes Ubiquitin-like protein SMT3. According to some studies, SMT3 may have the following features.
A new factor required for the SUMO1/Smt3 conjugation of yeast membrane proteins. The ubiquitin-like proteins SMT3 and SUMO-1 are bound by the UBC9 E2 enzyme. Yeast ULP2 (SMT4) gene encodes a new protease, which is specific for ubiquitin-like Smt3 protein. Yeast Ull1/Siz1 is a new type of SUMO1/Smt3 ligase, used for septin components, and acts as an adaptor between the ligase and the substrate. A new type of mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localizes to nucleosomes during interphase. Sterol methyltransferases SMT1, SMT2 and SMT3 affect the development of Arabidopsis thaliana through non-sterol products. The combination of Smt3 and Dorsal may enhance the immune response of fruit flies.