Product Details

Purity

>85% (SDS-PAGE)

Target Names

MSN2

Uniprot No.

P33748

Alternative Names

MSN2; YMR037C; YM9532.02C; Zinc finger protein MSN2; Multicopy suppressor of SNF1 protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Positive transcriptional factor that acts as a component of the stress responsive system. Recognizes and binds to the stress response element (STRE) which is involved in the response to various forms of stress (heat, oxidative, osmotic, etc.). Involved in the regulation of the CTT1, DDR2, HSP12 genes. May be regulated via WHI2-PSR1 complex phosphatase activity.

Gene References into Functions

Under non-stress conditions, stress-responsive transcriptional activator Msn2 is more abundant in the nucleus of the high tolerance spore clone. High tolerance to oxidative stress in this spore clone results from a high expression of reactive oxygen species-degrading enzymes by the abundant accumulation of Msn2 in the nucleus. PMID: 29607878
In this mutant lacking the Snf1-mediated positive feedback loop, Msn2 responds similarly to glucose limitation and osmotic stress, and its pulsatile translocation is largely abrogated. PMID: 28637875
Information transduction through amplitude and frequency regulation of Msn2 is limited to error-free transduction of signal identity, but not signal intensity information. PMID: 25985085
the environmental stress response in the yeast S. cerevisiae constitutes a system where gene activation is linearly commensurate with the cell's perception of environmental stress as encoded by the level of active Msn2. PMID: 24210615
A dynamic interplay between nucleosomes and Msn2 regulates gene expression in yeast stress response. PMID: 24598258
Data indicate that Msn2-GFP resides in the nucleus under unstressed conditions, but 80-90% accumulates in the nucleus after a severe stress. PMID: 23615444
AUthors identified new functional motifs in the Msn2 transcriptional-activating domain. PMID: 22505609
Msn2 acted as a tunable signal processor that could track, filter, or integrate signals in an input-dependent manner; this tunable signal processing appears to originate from dual regulation of both nuclear import and export by phosphorylation PMID: 23349292
Data show that the identities and intensities of different stresses are transmitted by modulation of the amplitude, duration or frequency of nuclear translocation of the general stress response transcription factor Msn2. PMID: 22179789
PP2A and the Tor kinase pathway transduce stress and nitrogen starvation signals to Msn2p. PMID: 15470255
increased degradation of nuclear Msn2 might be necessary to adapt cells to low PKA conditions after the diauxic shift or to allow growth under chronic stress conditions PMID: 15502160
The general stress responses of yeast genes are activated by Msn2. PMID: 15545648
Stress response transcription factors Msn2p/4p are repressed by protein kinase A. PMID: 15545649
localization determinants present in the N-terminal half of Msn2p are insensitive to variations in the intracellular cAMP concentrations PMID: 16489456
Genes regulated by Plc1p do not correlate with gene sets regulated by Swi/Snf or RSC chromatin remodeling complexes but show correlation with genes controlled by Msn2p. PMID: 18375619
stochastic version of the model for periodic nucleocytoplasmic shuttling of Msn2 coupled to oscillations in the cAMP-PKA pathway PMID: 18492651
In this review, Msn2 and Msn4 regulation during heat stress response is discussed. PMID: 19435282

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Subcellular Location

Cytoplasm. Nucleus.

Database Links

KEGG: sce:YMR037C

STRING: 4932.YMR037C

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Recombinant Saccharomyces cerevisiae Zinc finger protein MSN2 (MSN2), partial

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