Product Details

Purity

>85% (SDS-PAGE)

Target Names

MSN4

Uniprot No.

P33749

Alternative Names

MSN4; YKL062W; Zinc finger protein MSN4; Multicopy suppressor of SNF1 protein 4

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Positive transcriptional factor that acts as a component of the stress responsive system. Recognizes and binds to the stress response element (STRE) which is involved in the response to various forms of stress (heat, oxidative, osmotic, etc.). Involved in the regulation of the CTT1, DDR2, HSP12 genes.

Gene References into Functions

e transcription factors also play an important role in the exit of quiescence and regrowth. By studying Saccharomyces cerevisiae under a continuous, nutrient-limited condition, we found that Msn2 and Msn4 function as master regulators of glycolytic genes in the quiescent-like phase. PMID: 29455333
Thus Msn2/4 exhibit a dual role in activating carbohydrate metabolism genes and stress response genes. PMID: 28949295
Data suggest that promoters of most genes involved in biogenesis, function, and regulation of peroxisomes contain msn2- and msn4-binding sites; msn2 exhibits increased occupancy on promoters of beta-oxidation genes in glucose-depleted conditions; thus, msn2 and msn4 transcription factors activate expression of genes involved in fatty acid oxidation. PMID: 28924051
Target genes of Msn2 and Msn4 with fast activation kinetics can be fully induced by either factor, behaving as an 'OR' gate. In contrast, target genes with slow activation kinetics behave as an 'AND' gate, requiring distinct contributions from both factors, upon transient stimulation. PMID: 27690227
suppression of Msn2/Msn4 activity in the absence of stress is achieved by several independent control mechanisms, including Msn2/Msn4 phosphorylation and proteosome degradation PMID: 21757539
The anthocyanins delphinidin 3-glucoside and petunidin 3-glucoside were found to improve significantly the growth rate of Saccharomyces cerevisiae in an Msn2-, Msn4-dependent manner. PMID: 20846146
Crz1 destabilizes Msn2/Msn4 in the nuclei of cells in response to Ca2+ signalling. PMID: 20121702
This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. PMID: 19702872
role of Msn2 and Msn4 in mediating the transcriptional response of short term anaerobiosis PMID: 15870279
elevated pressure activates STRE dependent transcription through Msn2/4, which are also required for cell resistance and cell adaptation to high pressure. PMID: 17055490
The double deletion of MSN2 and MSN4 eliminates both chromatin remodeling and heat shock factor binding only at the HSP12 promoter, suggesting that Msn2/4 activators are primary determinants of chromatin disassembly at the HSP12 promoter. PMID: 18070923
NuA4 physically interacts with Msn4 but does not regulate Msn4 binding to the HSP12 promoter or heat shock induction of HSP12. PMID: 18212056
In this review, Msn2 and Msn4 regulation during heat stress is discussed. PMID: 19435282

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Subcellular Location

Cytoplasm. Nucleus.

Database Links

KEGG: sce:YKL062W

STRING: 4932.YKL062W

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Recombinant Saccharomyces cerevisiae Zinc finger protein MSN4 (MSN4), partial

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