Recombinant Salmonella typhimurium Flagellar hook-associated protein 2 (fliD)

Code CSB-YP321436SXB
MSDS
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Source Yeast
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Code CSB-EP321436SXB
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Source E.coli
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Code CSB-EP321436SXB-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321436SXB
MSDS
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Source Baculovirus
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Code CSB-MP321436SXB
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
fliD
Uniprot No.
Alternative Names
fliD; flaV; flbC; STM1960Flagellar hook-associated protein 2; HAP2; Filament cap protein; Flagellar cap protein
Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Expression Region
2-467
Target Protein Sequence
ASISSLGVG SNLPLDQLLT DLTKNEKGRL TPITKQQSAN SAKLTAYGTL KSALEKFQTA NTALNKADLF KSTVASSTTE DLKVSTTAGA AAGTYKINVT QLAAAQSLAT KTTFATTKEQ LGDTSVTSRT IKIEQPGRKE PLEIKLDKGD TSMEAIRDAI NDADSGIAAS IVKVKENEFQ LVLTANSGTD NTMKITVEGD TKLNDLLAYD STTNTGNMQE LVKAENAKLN VNGIDIERQS NTVTDAPQGI TLTLTKKVTD ATVTVTKDDT KAKEAIKSWV DAYNSLVDTF SSLTKYTAVE PGEEASDKNG ALLGDSVVRT IQTGIRAQFA NSGSNSAFKT MAEIGITQDG TSGKLKIDDD KLTKVLKDNT AAARELLVGD GKETGITTKI ATEVKSYLAD DGIIDNAQDN VNATLKSLTK QYLSVSNSID ETVARYKAQF TQLDTMMSKL NNTSSYLTQQ FTAMNKS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.
Gene References into Functions
  1. The solution structure of the FliT chaperone in the free state and in complex with FliD and the flagellar ATPase FliI has been determined. PMID: 27528687
  2. Escherichia coli FliD consists of three domains (D1, D2, and D3) and forms a hexamer plate of the D2 and D3 domains that resembles a six-pointed star with legs consisting of the D1 domain. In contrast, the D2 and D3 domains of Salmonella typhimurium FliD assemble into a pentamer as a five-pointed star plate. Despite their distinct oligomeric states, ecFliD and stFliD engage a common molecular surface for oligomerization. PMID: 28179186
Subcellular Location
Secreted. Bacterial flagellum.
Protein Families
FliD family
Database Links

KEGG: stm:STM1960

STRING: 99287.STM1960

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