Recombinant Schizosaccharomyces pombe Cleavage and termination factor 1 (ctf1)

Code CSB-YP529804SXV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP529804SXV
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP529804SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529804SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP529804SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ctf1
Uniprot No.
Alternative Names
ctf1; SPBC3B9.11cCleavage and termination factor 1; Transcription termination factor ctf1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-363
Target Protein Sequence
MSMTAGNVVF VGNIPYDVSE QQMTEIFNQV GPVKTFKLVL DPETGSGKGY GFCEFFDSET TAMAVRKLNN SELGPRKIRV EFPSNDPRRN QSYEYTERTD RYMEQQNAHE SSYNSRFIPP VLHSTSSLPA SQGGGMPSPA IYSSSMATNL NKNINSTSVP AYNFHNSMTS DFDSASQPHT DAYNARTFQY NKSSQNKGDY TSGTSISNPT SIPLAPSVVQ VLSTFSAQEL LNMLSKLQTV VHIAPEEARR LLIANPALPY AAFQAMLLMN LVDANVLQQV VVAVKNKNMH QPASATSSPP SVPQKIPSSN HKSQQANGSD QGNEGKRMAL IQQLLALTPE QINALPPAQR DQILSIRRQH FRQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.
Gene References into Functions
  1. The deletion of ctf1 gene affects the chromosomal attachment to the mitotic spindle leading to the accumulation of Bub1-GFP foci. Ctf1 localizes to the nucleus and physically interacts with Rna14, a cleavage and polyadenylation factor. PMID: 28567704
Subcellular Location
Nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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