Recombinant Schizosaccharomyces pombe Copper transport protein ctr4 (ctr4), partial

Code CSB-YP530961SXV1
MSDS
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Source Yeast
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Code CSB-EP530961SXV1
MSDS
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Source E.coli
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Code CSB-EP530961SXV1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP530961SXV1
MSDS
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Source Baculovirus
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Code CSB-MP530961SXV1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ctr4
Uniprot No.
Alternative Names
ctr4; SPCC1393.10; Copper transport protein ctr4; Copper transporter 4
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for high affinity copper (probably reduced Cu I) transport into the cell.
Gene References into Functions
  1. The authors propose that the Ctr4 central domain mediates Cu transport in this hetero-complex, whereas the Ctr5 carboxyl-terminal domains functions in the regulation of trafficking of the Cu transport complex to the cell surface. [Ctr4 & Ctr5] PMID: 21273250
  2. Ctr4 and Ctr5 proteins co-localize at the plasma membrane shortly after meiotic induction, whereas Ctr6 is located on the membrane of vacuoles. PMID: 24569997
  3. a copper-dependent internalization and recycling of the heteromeric Ctr4-Ctr5 complex PMID: 20694150
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Copper transporter (Ctr) (TC 1.A.56) family, SLC31A subfamily
Database Links
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