Recombinant Schizosaccharomyces pombe DNA repair protein rhp51 (rhp51)

Code CSB-YP330610SXV
MSDS
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Source Yeast
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Code CSB-EP330610SXV
MSDS
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Source E.coli
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Code CSB-EP330610SXV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330610SXV
MSDS
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Source Baculovirus
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Code CSB-MP330610SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rhp51
Uniprot No.
Alternative Names
rhp51; rad51; SPAC644.14c; DNA repair protein rhp51; RAD51 homolog
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-365
Target Protein Sequence
MADTEVEMQV SAADTNNNEN GQAQSNYEYD VNVQDEEDEA AAGPMPLQML EGNGITASDI KKIHEAGYYT VESIAYTPKR QLLLIKGISE AKADKLLGEA SKLVPMGFTT ATEYHIRRSE LITITTGSKQ LDTLLQGGVE TGSITELFGE FRTGKSQICH TLAVTCQLPI DMGGGEGKCL YIDTEGTFRP VRLLAVADRY GLNGEEVLDN VAYARAYNAD HQLELLQQAA NMMSESRFSL LVVDSCTALY RTDFSGRGEL SARQMHLARF MRTLQRLADE FGIAVVITNQ VVAQVDGISF NPDPKKPIGG NILAHSSTTR LSLRKGRGEQ RICKIYDSPC LPESEAIFAI NSDGVGDPKE IIAPV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double-stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.
Gene References into Functions
  1. Data show that Rad51-dependent homologous recombination (HR) predominates in centromeres. PMID: 28977643
  2. Rad51 and Rad54 are required for gene silencing in centromeres, suggesting that homologous recombination also plays a role in the structure and function of centromeres. PMID: 27697832
  3. spindle assembly checkpoint-dependent prometaphase arrest occurred more frequently in pot1Delta rqh1-hd double mutants than in rqh1-hd single mutants in Rad51-dependent recombination intermediates at telomeres PMID: 24469396
  4. Rad22-Mus81-dependent (Rhp51-independent) pathway is an important mechanism for the repair of DNA damage in fission yeast PMID: 15486206
  5. The recombinase Rad51 forms helical nucleoprotein filaments on single-stranded DNA. PMID: 15899844
  6. Rhp51 action is controlled through an interplay between Fbh1 and the mediator proteins.(Rhp51) PMID: 16135800
  7. Rad51 protein localizes to linear elements. PMID: 16532353
  8. genes rhp51+ and rhp55+ are involved not only in the repair of induced DNA double-strand breaks, but also in the mechanism of mating type switching in fission yeast. PMID: 16756067
  9. fission yeast (Rhp51) and human (hRad51) RecA homologues promote duplex-duplex DNA-strand exchange in vitro PMID: 18256600
  10. Rad22 (the S. pombe Rad52 homolog) and the Swi5-Sfr1 complex have distinct roles for mediating Rhp51 loading onto replication protein A-precoated ssDNA. PMID: 18416603
  11. These data suggest that Rad51 suppresses rearrangements of centromere repeats that result in isochromosome formation. PMID: 18923422

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Subcellular Location
Nucleus.
Protein Families
RecA family, RAD51 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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