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Recombinant Schizosaccharomyces pombe F-box DNA helicase protein 1 (fbh1), partial

Recombinant Schizosaccharomyces pombe F-box DNA helicase protein 1 (fbh1), partial

Code	CSB-YP892017SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP892017SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP892017SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP892017SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP892017SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

fbh1

Uniprot No.

Q9USU3

Alternative Names

fbh1; fdh; fdh1; SPBC336.01F-box DNA helicase protein 1; EC 3.6.4.12

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in ATP-dependent DNA-unwinding in a 3' to 5' direction, and ATP-ase activities stimulated by the single-stranded DNA-binding protein ssb1. Essential for viability and normal growth of stationary phase cells and in the absence of either srs2 or rqh1 DNA helicase. Involved in DNA recombination repair of strand breaks and stalled or collapsed replication forks, on the rhp51-dependent pathway: promotes rhp51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination. Ubiquitination and DNA helicase activities are essential for controlling rhp51-dependent recombination in the absence of rad22. Plays a role in the processing of toxic recombination intermediates. Promotes proper chromosome segregation.

Gene References into Functions

we tentatively suggest that Fbh1 might also have a pro-recombination role in vivo, because the Fbh1-Skp1 complex stimulated Rad51-mediated strand exchange in vitro PMID: 25165823
SCF/Skp1 together with Fbh1 is likely to function in the resolution of meiotic recombination intermediates, thereby ensuring proper chromosome segregation. PMID: 22292001
Fbh1 also serves a vital meiotic role in fission yeast to promote normal chromosome segregation. PMID: 21149262
a version of Atf1 lacking all intact MAPK sites still interacts with Fbh1 upon stress, indicating that the association between the F box protein and substrate is disrupted by stress-induced phosphorylation PMID: 19836238
The F-box DNA helicase Fbh1 functions in recombination repair in the Rhp51 pathway downstream of Rhp51 and Rhp57, and plays a role in processing recombination intermediates. [fbh1] PMID: 16135799
Mediator proteins are required to enable Rhp51 to function in the presence of Fbh1. Analysis of fbh1 mutant alleles suggests that both the F-box and helicase activities of Fbh1 contribute to controlling Rhp51 action. [fbh1] PMID: 16135800
Data show that the F-box DNA helicase (Fbh1) functions in direct opposition to the Rad52 orthologue Rad22 to curb Rad51 loading onto DNA in fission yeast. PMID: 19546232