Product Details

Purity

>85% (SDS-PAGE)

Target Names

smc6

Uniprot No.

P53692

Alternative Names

smc6; rad18; SPCC5E4.06; Structural maintenance of chromosomes protein 6; DNA repair protein rad18

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Plays a critical role in meiosis.

Gene References into Functions

Study shows that the Smc5/6-hinge complex binds preferentially to ssDNA and that this interaction is affected by both 'latch' and 'hub' mutations, suggesting a key role for these unique features in controlling DNA association by the Smc5/6 complex. PMID: 28134253
Results provide evidence that Brc1 provides scaffolding functions linking gammaH2A, Rhp18, and Smc5/6 complex at damaged replication forks. PMID: 28784724
The function of the SMC5/6 complex and its mammalian homologs in the cell cycle has not been clarified yet. PMID: 26743946
Smc6 is a required for homologous recombination in S. pombe. PMID: 21976700
Smc6 contributes to DNA repair in vivo by targeting the Smc5-6 complex to single-stranded DNA substrates created during the processes of homologous recombination and/or DNA replication. PMID: 22086171
In an examination of the evolutionary conservation of the Nse3-Nse4 interactions, we find that several MAGE proteins can interact with at least one of the NSE4/EID proteins. PMID: 21364888
there are two subcomplexes containing Rad18-Spr18-Nse2 and Nse1-Nse3-Rad62, which are probably bridged by a weaker interaction between Nse2 and Nse3 PMID: 15601840
analysis of bridging of the Smc5-Smc6 heads by the KLEISIN, Nse4, and non-Kleisin subunits in the Smc5-Smc6 DNA repair complex PMID: 17005570
When replication forks collapse, Smc6 (formerly known as rad18) is required for the effective repair of lesions but not for the recruitment of recombination proteins. PMID: 17030601
Brc1-mediated rescue of Smc5/6 deficiency: requirement for multiple nucleases and a novel Rad18 function. PMID: 17277362
Nse1 RING-like motif is a protein-protein interaction domain required for Smc5-Smc6 holocomplex integrity and recruitment to, or retention at, DNA lesions PMID: 18667531
Data suggest that localization of Smc5/6 to centromeres and telomeres requires heterochromatin and SUMO, respectively. PMID: 18923417
Smc5/6 is required to maintain stalled forks in a stable recombination-competent conformation primed for replication restart. PMID: 19158664
Data show that defective cohesin removal is a major determinant of the mitotic lethality of Smc5-Smc6 mutants. PMID: 19528228

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Subcellular Location

Nucleus. Chromosome.

Protein Families

SMC family, SMC6 subfamily

Database Links

KEGG: spo:SPCC5E4.06

STRING: 4896.SPCC5E4.06.1

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Recombinant Schizosaccharomyces pombe Structural maintenance of chromosomes protein 6 (smc6), partial

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