Recombinant Schizosaccharomyces pombe Uncharacterized homeobox protein C21B10.13c (SPBC21B10.13c)

Code CSB-YP328193SXV
MSDS
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Source Yeast
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Code CSB-EP328193SXV
MSDS
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Source E.coli
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Code CSB-EP328193SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328193SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP328193SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
yox1
Uniprot No.
Alternative Names
yox1; SPBC21B10.13c; MBF complex negative regulatory component yox1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-201
Target Protein Sequence
MSLSDSPSKS GNTGKDLISN NEAKNHEDEE THQKKRRRRT TDAEATLLEQ YFLKTPKPSL IERQELSKKL KSSMTPRELQ IWFQNKRQSL RRSNCLSRNR LEGTGENSLL RRKSTLTLCE TSTGQAELFF QSWPLHSQSV VGEMIHHEQD DYNKENKQQK VVDTTKDISR GSNGNEDSAA HQELEECARS LVELQQQCND H
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Negative regulatory component of the MBF transcription factor complex involved in cell-cycle G1/S phase-specific gene expression and more particularly DNA replication checkpoint-dependent gene expression.
Gene References into Functions
  1. data show that Yox1 acts to co-ordinate the expression of MBF-regulated genes during the cell division cycle; data suggests that Yox1 is inactivated by the Rad3 DNA replication checkpoint via phosphorylation by the conserved Cds1 checkpoint kinase PMID: 21304269
  2. Checkpoint-dependent phosphorylation of Yox1p at Ser114, Thr115 results in de-repression of the MBF transcriptional program. PMID: 21359180
  3. phosphorylation of Yox1 when the checkpoint kinase is activated triggers the transcriptional induction of the S-phase genes PMID: 21132016
  4. Yox1 as a new regulatory MBF component in fission yeast, which is transcriptionally induced by MBF and in turn inhibits MBF-dependent transcription. PMID: 19714215
Subcellular Location
Nucleus.
Database Links
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