Recombinant Schizosaccharomyces pombe Vacuolar protein sorting-associated protein 74 (vps74)

Code CSB-YP521111SXV
MSDS
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Source Yeast
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Code CSB-EP521111SXV
MSDS
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Source E.coli
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Code CSB-EP521111SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP521111SXV
MSDS
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Source Baculovirus
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Code CSB-MP521111SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
vps74
Uniprot No.
Alternative Names
vps74; SPAC5D6.13; SPAPJ735.02c; Vacuolar protein sorting-associated protein 74
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-337
Target Protein Sequence
MSGGLSRRRV AAASSDEERP EMRSVSHSKS HLEGYDDDHK IAFDPKDLEQ GAEREKQPRL TLMEEVLLLG LKDKQGYLSF WNDSISYSLR GCILMELAFR GKLRMQKDVN RKRFPLADRV VELVDEKLTG EVLLDEAIKM IHTSELMSVT SWIDLMSGET WNVMKIGYQL RQVRERLAKG LVDKGILRNE KKNFLLFDMP THPIADTSIK DSIKKRVVSV LTSRVIEIDN SPSFPEYLSF RCLRTVALAC SSYAANVLEN ALDNLNYEDR EKAFSRVDEL LCDFSQWPFD LHASSSVGAN LPELISEELS SVKEEQLLFI EVVAAVLQVF THLDSLL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.
Subcellular Location
Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
GOLPH3/VPS74 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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