Product Details

Purity

>85% (SDS-PAGE)

Target Names

mmi1

Uniprot No.

O74958

Alternative Names

mmi1; SPCC736.12c; YTH domain-containing protein mmi1; Meiotic mRNA interception protein 1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Expression Region

1-488

Target Protein Sequence

MSNTNFSTSR SSKSIPELPN LEALRSLWPP PSLNESGDTR SVWTTHTGEP VASSVLSTSG SNNFSSPLKR PAPESHDAPI GRRLMVDDPR LIKHGKYDFS RHCTDYGHSY EWPYFRSLRR ESMLYHTSGS YPESQPPYSS YSTDAPHYYH AGSESSAYYD SRSRLHGIQP PPKRRTLSPP PRRLADPVVV GSSRYVEEEV YRRPPYTLAS EVPSSASAYQ AGYSSYPVRS SPQLSHEDTR HGIASSGSTR YPFVPANTRA SHSPSLLEPY AHSLPSSVAP VGAYPEKSSY LLSNSSNDSA SRKEKPKARA STPPPLNFSR ASEHRNEKGE RISMINPRVV LDENGISHRS RYFIMLCDNE TAIAHAKKTS IWAVKKDSSK RISDAYKKAS VYFIFVAQQT YNALGYAQVV SDLNSTELPF WSDSSHAGGV RIKWIKTCNL FSAEISEIVS HMDHGSEARD GMEMMYDEGS RLCTLINYAI MKRIGRDR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs. Functions alone and as part of the erh1-mmi1 complex, to recruit the CCR4-NOT complex and the NURS complex to target RNAs. Suppresses the meiotic program during vegetative growth and promotes the meiotic program during mating. Binds to DSR (determinant of selective removal) regions in meiotic mRNA, and recruits the NURS complex to targets. Recruitment of NURS complex to target mRNAs promotes mRNA decay by engagement of the nuclear exosome, and formation of heterochromatin islands at meiotic genes silenced by the exosome. Recruitment of the CCR4-NOT complex to target RNAs promotes heterochromatin formation at RNAi-dependent heterochromatin domains (HOODs), including a subset of meiotic genes, lncRNAs and retrotransposons. Recruitment of the CCR4-NOT complex to rDNA promotes rDNA heterochromatin assembly. Promotes non-canonical transcription termination at meiotic genes and prevents lncRNA transcription from invading and repressing adjacent genes.

Gene References into Functions

The authors propose that Mmi1 recruits the Ccr4-Not complex to counteract its own inhibitor Mei2, thereby locking the system in a stable state that ensures the repression of the meiotic program by Mmi1. PMID: 28841135
Mmi1 can recognize and bind a class of meiosis-specific transcripts expressed inappropriately in mitotic cells; structural insights into the specific recognition of these mRNAs by the YTH domain containing protein Mmi1 have been presented. PMID: 28735863
report the 1.75 A crystal structure of the Mmi1 YTH domain and provide evidence that Mmi1 recognizes DSR RNA via a binding mode distinct from that of structurally homologous YTH proteins that recognize m(6)A-modified RNA PMID: 27165520
Erh1 and the RNA-binding protein Mmi1 form a stoichiometric complex, called the Erh1-Mmi1 complex (EMC), to promote meiotic mRNA decay and facultative heterochromatin assembly. PMID: 26942678
Mmi1 YTH domain selectively recognizes the determinant of selective removal RNA motif. PMID: 26673708
Mmi1 can specifically regulate the splicing of particular introns in a transcript: it inhibits the splicing of introns that are in the vicinity of putative Mmi1 binding sites, while allowing the splicing of other introns that are far from such sites. PMID: 22046364
Mei2 tethers Mmi1 and thereby stabilizes the transcripts necessary for the progression of meiosis PMID: 20948174

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Subcellular Location

Nucleus.

Database Links

KEGG: spo:SPCC736.12c

STRING: 4896.SPCC736.12c.1

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Recombinant Schizosaccharomyces pombe YTH domain-containing protein mmi1 (mmi1)

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