Recombinant Serine/threonine-protein kinase sgk-1 (sgk-1)

Code CSB-YP650826CXY
MSDS
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Source Yeast
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Code CSB-EP650826CXY
MSDS
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Source E.coli
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Code CSB-EP650826CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP650826CXY
MSDS
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Source Baculovirus
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Code CSB-MP650826CXY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sgk-1
Uniprot No.
Alternative Names
sgk-1; W10G6.2; Serine/threonine-protein kinase sgk-1; EC 2.7.11.1; Serum- and glucocorticoid-inducible kinase homolog; Serum/glucocorticoid-regulated kinase 1
Species
Caenorhabditis elegans
Expression Region
1-463
Target Protein Sequence
MGSMYYNESR MVRKDEVTCN VIIGDDKKTV VYALRIGNGP IMQKTFEEYE RFFTTEKDMI PATIFTAPKK KFLQADSKFY EKRRVWILVI SQHLVDNNLR SEDVRRFFHL ESPDDDENNV DLGPSERKTA TANDFDYLTT IGKGSFGRVY QVRHKETKKI YAMKILSKEH IRKKNEVKHV MAERNVLINN FKHPFLVSLH FSFQNKEKLY FVLDHLNGGE LFSHLQREKH FSESRSRFYA AEIACALGYL HEKNIIYRDL KPENLLLDDK GYLVLTDFGL CKEDMQGSKT TSTFCGTPEY LAPEIILKKP YDKTVDWWCL GSVLYEMIFG LPPFYSKDHN EMYDKIINQP LRLKHNISVP CSELITGLLQ KDRSKRLGHR NDFRDIRDHP FFLPVDWDKL LNRELKAPFI PKVKNAMDTS NISKEFVEIQ IDPSSLAPQQ LAVTHRDHDF ENFTFVDTNR VLV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts downstream of PI3 kinase age-1 and kinase pdk-1 in the daf-2/insulin receptor-like transduction pathway. Essential role in regulating development, stress response, and longevity. Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their function. Promotes the cytoplasmic localization of the transcription factor pqm-1. Plays a role in the intracellular trafficking of proteins such as mig-14 to the cell membrane, and this may be through positively regulating ceramide synthesis. Acts downstream of rict-1 to regulate fat storage, size, development and vitellogenesis. Downstream of age-1 and together with akt-1/2, promotes cell survival during embryonic development. Does not appear to play a role in immune function.
Gene References into Functions
  1. SGK-1 acts in parallel to the AKT kinases to mediate DAF-2 signaling. Loss of sgk-1 results in defective egg-laying, extended generation time, increased stress resistance, and an extension of life span. PMID: 15068796
  2. findings indicate that SGK-1 is a mediator of Rictor activity; findings identify new physiological roles for TORC2, mediated by SGK, in regulation of C. elegans lipid accumulation and growth PMID: 19260765
Subcellular Location
Cytoplasm. Nucleus. Apical cell membrane.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family
Tissue Specificity
Expressed in late embryos just before hatching. At postembryonic stages, expressed in sensory and motor neurons and in the intestine. Highly expressed in the intestine and head and tail neurons.
Database Links

KEGG: cel:CELE_W10G6.2

STRING: 6239.W10G6.2b

UniGene: Cel.19781

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