Recombinant Serine/threonine-protein kinase unc-51 (unc-51), partial

Code CSB-YP635495CXY
MSDS
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Source Yeast
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Code CSB-EP635495CXY
MSDS
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Source E.coli
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Code CSB-EP635495CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP635495CXY
MSDS
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Source Baculovirus
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Code CSB-MP635495CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
unc-51
Uniprot No.
Alternative Names
unc-51; Y60A3A.1; Serine/threonine-protein kinase unc-51; EC 2.7.11.1; Uncoordinated protein 51
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein kinase important for axonal elongation and axonal guidance. Functions in the CAN axons to direct both anterior and posterior migrations. Phosphorylates both unc-14 and vab-8. Component of the unc-51/atg-13 complex that is probably recruited by lgg-1 to preautophagosomes and is required for autophagosome formation. Interaction with autophagy related proteins such as atg-13 links it to the autophagy machinery to in turn promote P-granule degradation in somatic cells. Plays a role in mitophagy during limited food availability. Regulates cell size. Plays a role in male tail ray pattern formation. May be required for normal dauer morphogenesis.
Gene References into Functions
  1. Mutants with impaired growth cone functions, such as unc-51, often exhibit abnormal terminations and inappropriate trajectories at the distal ends of the M2 axons. PMID: 12885562
  2. The autophagy-related kinase UNC-51 regulates the subcellular localization of the Netrin receptor UNC-5 in Caenorhabditis elegans PMID: 16887826
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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