Product Details

Purity

>85% (SDS-PAGE)

Target Names

env

Uniprot No.

P31621

Alternative Names

envEnvelope glycoprotein; Env polyprotein) [Cleaved into: Surface protein; SU; Glycoprotein 52; gp52); Transmembrane protein; TM; Glycoprotein 36; gp36)]

Species

Sheep pulmonary adenomatosis virus (Jaagsiekte sheep retrovirus) (JSRV)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

The envelope proteins induce cell transformation leading to ovine pulmonary adenocarcinoma (OPA), a contagious lung cancer of sheep and goat. They bind to the HYAL2 receptor for cell entry. Env proteins probably do not act as oncogenes by themselves, but may rather liberate an oncogenic factor that would normally be negatively regulated. One mechanism of transformation seems to involve activation of the phosphoinositide-3-OH kinase (PI3K)/Akt pathway but does not involve the virus receptor HYAL2, and the other seems to involve Env binding to HYAL2, HYAL2 degradation, and activation of the MST1R receptor tyrosine kinase, which is normally suppressed by HYAL2.

Gene References into Functions

BST2B acts differently from BST2A by leading to a reduction in Jaagsiekte sheep retrovirus Env incorporation into viral particles, which ultimately results in the release of virions that are less infectious. PMID: 25339764
study reveals critical determinants in SU subunits of JSRV and ENTV Env proteins that likely regulate their local structure and thereby differential receptor-mediated fusion activation at low pH; findings explain, in part, their distinct viral infectivity PMID: 22018783
The authors observed that Env of Jaagsiekte sheep retrovirus induced the expression of a tumor suppressor, Sprouty2, suggesting a correlation between Env-effect and Sprouty2 expression. PMID: 20678191
These data strongly suggest that the JSRV Env functions as a dominant oncoprotein in the natural immunocompetent host and that JSRV can induce ovine pulmonary adenocarcinoma in the absence of viral spread. PMID: 16873259
The role of the env transmembrane cytoplasmic tail in JSRV transformation was studied. PMID: 16873263
JSRV Env protein is capable of inducing tumors in transgenic mice, and in other cell types besides lung epithelial cells. PMID: 17043760
signal peptide of the JSRV Env (JSE-SP) is 80 amino acid residues in length and contains putative nuclear localization and export signals, in addition to an arginine-rich RNA binding motif PMID: 19244321
In this study, the authors found that the betaretrovirus Jaagsiekte sheep retrovirus (JSRV) encodes within the env gene a trans-acting factor (Rej) necessary for the synthesis of Gag protein from unspliced viral RNA. PMID: 19776124
Rej response element-containing RNA relocalized Rej protein from the nucleus to the cytoplasm in 293 and rat 208F cells, suggesting binding of Rej to the Rej response element. PMID: 19776134

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Subcellular Location

[Surface protein]: Virion membrane; Peripheral membrane protein.; [Transmembrane protein]: Virion membrane; Single-pass type I membrane protein.

Database Links

KEGG: vg:1490021

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Recombinant Sheep pulmonary adenomatosis virus Envelope glycoprotein (env), partial

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