Recombinant Spermatogenesis-defective protein 39 (spe-39), partial

Code CSB-YP633812CXY
MSDS
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Source Yeast
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Code CSB-EP633812CXY
MSDS
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Source E.coli
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Code CSB-EP633812CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP633812CXY
MSDS
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Source Baculovirus
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Code CSB-MP633812CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
spe-39
Uniprot No.
Alternative Names
spe-39; vipar; ZC404.3; Spermatogenesis-defective protein 39; SPE-39; VPS33B-interacting protein in polarity and apical restriction
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Proposed to be involved in endosomal maturation implicating in part vps-33.2. May play a role in epithelial polarization through stabilization of apical membrane protein content. May play a role in transcriptional regulation. Plays a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes. Essential for vesicular trafficking during spermatogenesis; required during spermiogenesis or sperm activation for the morphogenesis of specialized Golgi-derived fibrous body-membranous organelle (FB-MO) complexes. Required for the processing of internalized proteins in oocytes and coelomocytes.
Gene References into Functions
  1. SPE-39 homologues are present in RAB5-, RAB7-, and RAB11-positive endosomes where they play a conserved role in lysosomal delivery and probably function via their interaction with the core HOPS complex. PMID: 19109425
Subcellular Location
Cytoplasm. Cytoplasmic vesicle. Early endosome. Recycling endosome. Late endosome.
Protein Families
SPE39 family
Tissue Specificity
Expressed in spermatocytes and spermatids (at protein level).
Database Links

KEGG: cel:CELE_ZC404.3

STRING: 6239.ZC404.3a

UniGene: Cel.17954

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