Recombinant Staphylococcus aureus Clumping factor A (clfA), partial

The expression of recombinant Staphylococcus aureus clfA protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA encodes the 228-558aa of the Staphylococcus aureus clfA protein. This N-terminal 6xHis-tagged recombinant Staphylococcus aureus clfA protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant clfA protein migrated to the band of about 36 kDa molecular weight.

ClfA is a virulence factor from Staphylococcus aureus. As a virulence factor, ClfA is an important virulence factor that promotes the pathogenesis of the diseases and can be a target for generation of vaccine against S. aureus infections. As the targeted protein is highly antigenic and conserved, hence the designed novel vaccine construct holds potential against emerging multi-drug-resistant organisms. Genetic elimination of the binding motif on fibrinogen for the S. aureus virulence factor ClfA improves host survival in septicemia. B. There are many distinct virulence factors related to the disease establishment. In Staphylococcus spp. factors such as the fibronectin-binding proteins (fnbA and fnbB), elastin binding proteins (ebpS), clumping factors (clfA and clfB), and collagen-binding protein (cna) play important roles in binding to host cells, colonization, and invasion. Sortase A is an essential virulence factor of S. aureus, which is responsible for the determinants, which can escape host immune response and cause a series of diseases. SrtA mediates anchoring of several adhesion-related proteins, such as ClfA/ClfB.