clfA Antibody

Code CSB-PA753416ZA01SKW
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: clfA antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 52.1 kDa
    Observed band size: 54 kDa
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Product Details

Full Product Name
Rabbit anti-Staphylococcus aureus (strain MSSA476) clfA Polyclonal antibody
Uniprot No.
Target Names
clfA
Alternative Names
clfA antibody; SAS0752 antibody; Clumping factor A antibody; Fibrinogen receptor A antibody; Fibrinogen-binding protein A antibody
Raised in
Rabbit
Species Reactivity
Staphylococcus aureus
Immunogen
Recombinant Staphylococcus aureus Clumping factor A (clfA), partial (228-558AA)
Immunogen Species
Staphylococcus aureus (strain MSSA476)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The creation of the clfA polyclonal antibody is a meticulously structured process. It initiates with the repeated immunization of a rabbit using recombinant Staphylococcus aureus clfA protein (228-558aa), continuing until the desired antibody titer is attained. Following this, the rabbit's blood is collected, and the antibodies are purified from the serum with precision using protein A/G. The resulting clfA antibody is subjected to thorough assessment through ELISA and WB applications, confirming its specific binding with the Staphylococcus aureus clfA protein.

The clfA is a major virulence factor of Staphylococcus aureus. It plays a significant role in the bacterium's pathogenicity by facilitating adherence to host tissues, promoting bacterial clumping, and helping the bacterium evade host immune responses.

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Target Background

Function
Cell surface-associated protein implicated in virulence. Promotes bacterial attachment exclusively to the gamma-chain of human fibrinogen. Induces formation of bacterial clumps.
Subcellular Location
Secreted, cell wall; Peptidoglycan-anchor.
Protein Families
Serine-aspartate repeat-containing protein (SDr) family
Database Links

KEGG: sas:SAS0752

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