Recombinant Staphylococcus aureus Enterotoxin type B (entB)

Full-length of mature Staphylococcus aureus Enterotoxin type B (entB) cDNA carrying an N-terminal 6xHis-tag was expressed in the yeast cells. The resulting protein is the recombinant fusion protein consisting of 28-266aa of Staphylococcus aureus entB. The SDS-PAGE showed an about 28-33 kDa molecular mass of this protein and determined its purity of over 90%. In-stock entB proteins are offered now. In addition to producing specific anti-entB antibodies, this recombinant entB protein may also find uses in the microbiology.

Staphylococcus aureus-secreted exotoxin entB is one of the most potent bacterial superantigens (SAgs) that exerts toxic effects upon the immune system, inducing cytokine release and eliciting inflammation. EntB mainly targets T-cell receptors (TCRs) on the T cells and major histocompatibility complex (MHC) class II molecules on Ag-presenting cells (APCs), forming a ternary complex between specific Vβ chains and MHC class II molecules through cross-link. It is water-soluble, heat unstable, and resistant to the proteolytic enzyme, including trypsin, papain, and pepsin. And it also possesses emetic activity. EntB can cause multi-organ system failure and death at low concentrations. The involvement of entB in the pathogenesis of some diseases such as atopic dermatitis, asthma, and chronic rhinitis, has been documented.