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Recombinant Staphylococcus aureus Enterotoxin type E (entE)

Recombinant Staphylococcus aureus Enterotoxin type E (entE)

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Code	CSB-EP320170FKZ
Abbreviation	Recombinant Staphylococcus aureus entE protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

entE

Uniprot No.

P12993

Research Area

Others

Alternative Names

entEEnterotoxin type E; SEE

Species

Staphylococcus aureus

Source

E.coli

Expression Region

28-257aa

Target Protein Sequence

SEEINEKDLRKKSELQRNALSNLRQIYYYNEKAITENKESDDQFLENTLLFKGFFTGHPWYNDLLVDLGSKDATNKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWIDGKQTTVPIDKVKTSKKEVTVQELDLQARHYLHGKFGLYNSDSFGGKVQRGLIVFHSSEGSTVSYDLFDAQGQYPDTLLRIYRDNKTINSENLHIDLYLYTT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

42.4kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Dive into the study of bacterial pathogenesis with the Recombinant Staphylococcus aureus entE. Known as the Enterotoxin type E (SEE), this bacterial protein plays a crucial role in the infectious disease process. With origins in the notorious Staphylococcus aureus, this enterotoxin aids in understanding the host-pathogen interactions, superantigenicity, and food poisoning events associated with the bacteria.

The product comprises the mature form of the protein, incorporating amino acids 28-257, which represent the functionally vital region of the native protein. Expressed in an E.coli system, the product carries an N-terminal 6xHis-SUMO tag, facilitating ease in purification and detection. The purity of the Recombinant Staphylococcus aureus entE exceeds 90% as validated by SDS-PAGE. You have the flexibility to choose between a liquid form or a lyophilized powder based on your experiment's requirements. Harness the power of this enterotoxin to gain deeper insights into bacterial infectious diseases.

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Target Background

Function

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of proinflammatory cytokines. Causes also the intoxication staphylococcal food poisoning syndrome.

Subcellular Location

Secreted.

Protein Families

Staphylococcal/streptococcal toxin family

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