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Recombinant Staphylococcus aureus Enterotoxin type E (entE)

Recombinant Staphylococcus aureus Enterotoxin type E (entE)

Citations (1)

Code	CSB-YP320170FKZ
Abbreviation	Recombinant Staphylococcus aureus entE protein
MSDS	MSDS Datasheet
Size	US$436
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP320170FKZ could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus entE. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP320170FKZ could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus entE.
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

entE

Uniprot No.

P12993

Research Area

Cell Biology

Alternative Names

entEEnterotoxin type E; SEE

Species

Staphylococcus aureus

Source

Yeast

Expression Region

28-257aa

Target Protein Sequence

SEEINEKDLRKKSELQRNALSNLRQIYYYNEKAITENKESDDQFLENTLLFKGFFTGHPWYNDLLVDLGSKDATNKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWIDGKQTTVPIDKVKTSKKEVTVQELDLQARHYLHGKFGLYNSDSFGGKVQRGLIVFHSSEGSTVSYDLFDAQGQYPDTLLRIYRDNKTINSENLHIDLYLYTT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.4kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

A DNA sequence corresponding to 28-257aa of the Staphylococcus aureus Enterotoxin type E (entE) was expressed with an N-terminal 6xHis-tag in the yeast. The resulting protein is a full-length of the mature entE protein. It underwent the validation of LC-MS/MS analysis. Its purity reaches up to 90% determined by SDS-PAGE. It can be used to produce specific antibodies. This recombinant entE protein may also find uses in the studies of entE-associated cell biology.

As one of the most important microorganisms, Staphylococcus aureus causes various human diseases by secreting virulence factors staphylococcal superantigens (SAgs). EntE, also known as SEE, is one of the toxic SAgs, which can cross-link the T cell receptor (TCR) and major histocompatibility complex class II, triggering a massive T cell activation and hence disease. Depending on the dose and route of exposure, SEE and other SEs can cause food poisoning, acute and fatal respiratory distress, and toxic shock.

Citations

The CTLA‐4 immune checkpoint protein regulates PD‐L1: PD‐1 interaction via transendocytosis of its ligand CD80 A Kennedy,The EMBO journal,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western blot analysis

Review: DG-75 in the presence of 20 ng/ml Staphylococcus aureus Enterotoxin type E for the indicated time points to permit transendocytosis.

By Anonymous

Target Background

Function

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of proinflammatory cytokines. Causes also the intoxication staphylococcal food poisoning syndrome.

Subcellular Location

Secreted.

Protein Families

Staphylococcal/streptococcal toxin family

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